Cytotoxic T cells specific for antigens expressed on surface immunoglobulin-positive cells

J. Forman, R. Ciavarra, E. S. Vitetta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

C.B-20 mice were immunized with splenocytes or B leukemia cells (BCL1) from Ig H chain allotype congenic strains. Spleen cells from these immunized mice were rechallenged in vitro to generate H-2-restricted cytotoxic T cells that were specific for target antigens controlled by genes linked to the Ig H chain locus. The anti-Ig H cytotoxic T cells detected an antigen(s) expressed only in surface Ig+ cells. Thus, T cell lymphoblasts, eight BALB/c myeloma cells lines, and a T cell lymphoma were not lysed by the effector cells. In contrast, B cell lymphoblasts and the surface Ig+ BCL1 cells were sensitive to lysis. A surface Ig- hybridoma (which secretes the IgM from the BCL1 cells) generated by fusing BCL1 cells to X63 myeloma cells was not killed by the effector cells. These data indicate that cytotoxic T cells specific for antigenic determinants on either surface IgM or IgD or on a molecule that is coordinately expressed on IgM+ or IgD+ cells can be generated and that such cells might play a role in regulating the growth of normal B cells or surface Ig+ tumor cells in vivo.

Original languageEnglish (US)
Pages (from-to)1357-1368
Number of pages12
JournalJournal of Experimental Medicine
Volume154
Issue number5
StatePublished - 1981

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B-Cell Antigen Receptors
T-Lymphocytes
Antigens
Immunoglobulin M
Immunoglobulin D
B-Lymphocytes
B-Cell Leukemia
T-Cell Lymphoma
Hybridomas
Epitopes
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Cytotoxic T cells specific for antigens expressed on surface immunoglobulin-positive cells. / Forman, J.; Ciavarra, R.; Vitetta, E. S.

In: Journal of Experimental Medicine, Vol. 154, No. 5, 1981, p. 1357-1368.

Research output: Contribution to journalArticle

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N2 - C.B-20 mice were immunized with splenocytes or B leukemia cells (BCL1) from Ig H chain allotype congenic strains. Spleen cells from these immunized mice were rechallenged in vitro to generate H-2-restricted cytotoxic T cells that were specific for target antigens controlled by genes linked to the Ig H chain locus. The anti-Ig H cytotoxic T cells detected an antigen(s) expressed only in surface Ig+ cells. Thus, T cell lymphoblasts, eight BALB/c myeloma cells lines, and a T cell lymphoma were not lysed by the effector cells. In contrast, B cell lymphoblasts and the surface Ig+ BCL1 cells were sensitive to lysis. A surface Ig- hybridoma (which secretes the IgM from the BCL1 cells) generated by fusing BCL1 cells to X63 myeloma cells was not killed by the effector cells. These data indicate that cytotoxic T cells specific for antigenic determinants on either surface IgM or IgD or on a molecule that is coordinately expressed on IgM+ or IgD+ cells can be generated and that such cells might play a role in regulating the growth of normal B cells or surface Ig+ tumor cells in vivo.

AB - C.B-20 mice were immunized with splenocytes or B leukemia cells (BCL1) from Ig H chain allotype congenic strains. Spleen cells from these immunized mice were rechallenged in vitro to generate H-2-restricted cytotoxic T cells that were specific for target antigens controlled by genes linked to the Ig H chain locus. The anti-Ig H cytotoxic T cells detected an antigen(s) expressed only in surface Ig+ cells. Thus, T cell lymphoblasts, eight BALB/c myeloma cells lines, and a T cell lymphoma were not lysed by the effector cells. In contrast, B cell lymphoblasts and the surface Ig+ BCL1 cells were sensitive to lysis. A surface Ig- hybridoma (which secretes the IgM from the BCL1 cells) generated by fusing BCL1 cells to X63 myeloma cells was not killed by the effector cells. These data indicate that cytotoxic T cells specific for antigenic determinants on either surface IgM or IgD or on a molecule that is coordinately expressed on IgM+ or IgD+ cells can be generated and that such cells might play a role in regulating the growth of normal B cells or surface Ig+ tumor cells in vivo.

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