Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains

Iwona Stroynowski, Anders Orn, Robert S. Goodenow, Minnie McMillan, James Forman, Peter R. Brayton, Jeffrey Frelingers, Leroy Hood

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Abstract

We have shown that cytotoxic T lymphocytes (CTL) raised in H-2dmice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2Ld/Ddgene encoding a hybrid antigen with α1 and α2 external domains of H-2Ld and α3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV- and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the α3 domain of H-2Ld fused to α1 and α2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the α1 and/or α2 domains of the H-2Ld molecule.

Original languageEnglish (US)
Pages (from-to)141-154
Number of pages14
JournalImmunogenetics
Volume20
Issue number2
DOIs
StatePublished - Aug 1984

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Cytotoxic T-Lymphocytes
Lymphocytic choriomeningitis virus
Antigens
Viruses
Hybrid Cells
Virus Diseases
Genes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

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Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains. / Stroynowski, Iwona; Orn, Anders; Goodenow, Robert S.; McMillan, Minnie; Forman, James; Brayton, Peter R.; Frelingers, Jeffrey; Hood, Leroy.

In: Immunogenetics, Vol. 20, No. 2, 08.1984, p. 141-154.

Research output: Contribution to journalArticle

Stroynowski, Iwona ; Orn, Anders ; Goodenow, Robert S. ; McMillan, Minnie ; Forman, James ; Brayton, Peter R. ; Frelingers, Jeffrey ; Hood, Leroy. / Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by α1 and α2 but not α3 external domains. In: Immunogenetics. 1984 ; Vol. 20, No. 2. pp. 141-154.
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abstract = "We have shown that cytotoxic T lymphocytes (CTL) raised in H-2dmice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2Ld/Ddgene encoding a hybrid antigen with α1 and α2 external domains of H-2Ld and α3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV- and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the α3 domain of H-2Ld fused to α1 and α2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the α1 and/or α2 domains of the H-2Ld molecule.",
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