Cytotoxicity evaluation of methacrylate-based resins for clinical endodontics in vitro.

Esteban G. Garza, Aniket Wadajkar, Chul Ahn, Qiang Zhu, Lynn A. Opperman, Larry L. Bellinger, Kytai T. Nguyen, Takashi Komabayashi

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

This study examines the cytotoxicity of Super-Bond C&B (SB-C&B), Super-Bond RC Sealer (SB-RC), MetaSEAL (Meta), and AH Plus Sealer (AH+). Freshly mixed and set materials (100 mg) were prepared in vitro and placed in cell culture medium (1 mL) for the working time and for 6 h, respectively. L929 cells seeded into 96-well plates at 5,000 cells/well were incubated with the eluted medium (200 μL) for 24 h. Cells cultured with medium alone served as the control. Cytotoxicity was evaluated by MTS assay and analyzed with ANOVA. In the freshly mixed group, the average ± SD (%) for cell viability were 66.0 ± 13.6, 55.5 ± 15.6, 10.6 ± 0.7, and 8.9 ± 2.2 for SB-C&B, SB-RC, Meta, and AH+, respectively. In the set group, the average ± SD (%) for cell viability were 100 ± 21.9, 81.8 ± 38.5, 24.9 ± 7.9, and 23.6 ± 10.0 for SB-C&B, SB-RC, Meta, and AH+, respectively. SB-C&B and SB-RC are less cytotoxic than are Meta and AH+.

Original languageEnglish (US)
Pages (from-to)213-217
Number of pages5
JournalJournal of Oral Science
Volume54
Issue number3
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Dentistry(all)

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    Garza, E. G., Wadajkar, A., Ahn, C., Zhu, Q., Opperman, L. A., Bellinger, L. L., Nguyen, K. T., & Komabayashi, T. (2012). Cytotoxicity evaluation of methacrylate-based resins for clinical endodontics in vitro. Journal of Oral Science, 54(3), 213-217. https://doi.org/10.2334/josnusd.54.213