Cytotoxicity of a novel anti-ICAM-1 immunotoxin on human myeloma cell lines

Y. W. Huang, F. J. Burrows, E. S. Vitetta

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have generated a murine monoclonal antibody (UV3) which recognizes an epitope on ICAM-1 expressed on myeloma cells. By flow cytometric analysis, the epitope on ICAM-1 recognized by this antibody is strongly expressed on human myeloma cells, pre-B leukemia cells and Burkitt's lymphoma cell lines. Most human T cell lines are weakly positive. The antibody does not react with red blood cells, polymorphonuclear leukocytes (PMNs) or resting B lymphocytes from normal donors, and reacts very weakly with resting T cells. Immunohistochemical assays indicate that the antibody does not react with normal liver, kidney, heart, brain, thymus or lung. An immunotoxin (IT) was prepared by coupling UV3 to deglycosylated ricin A-chain (dgA). In protein synthesis inhibition assays it was highly cytotoxic to the human myeloma cell lines HS-SULTAN (IC50 = 1 x 10-11M) and ARH-77 (IC50 = 9 x 10- 11M), but not to cell lines of T cell lineage or most cell lines of the B lineage. Our results suggest that the UV3-dgA may have therapeutic potential for the treatment of human multiple myeloma.

Original languageEnglish (US)
Pages (from-to)661-675
Number of pages15
JournalHybridoma
Volume12
Issue number6
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Immunology
  • Genetics

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