D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain

Lily Jun Shen Huang, Kyle Durick, Joshua A. Weiner, Jerold Chun, Susan S. Taylor

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Subcellular localization directed by specific A kinase anchoring Proteins (AKAPs) is a mechanism for compartmentalization of cAMP-dependent protein kinase (PKA). Using a two-hybrid screen, a novel AKAP was isolated. Because it interacts with both the type I and type II regulatory subunits, it was defined as a dual specific AKAP or D-AKAP1. Here we report the cloning and characterization of another novel cDNA isolated from that screen. This new member of the D-AKAP family, D-AKAP2, also hinds both types of regulatory subunits. A message of 5 kb pairs was detected for D-AKAP2 in all embryonic stages and in all adult tissues tested. In brain, skeletal muscle, kidney, and testis, a 10-kb mRNA was identified. In testis, several small mRNAs were observed. Therefore, D-AKAP2 represents a novel family of proteins. cDNA cloning from a mouse testis library identified the full length D-AKAP2. It is composed of 372 amino acids which includes the R binding fragment, residues 333-372, at its C-terminus. Based on coprecipitation assays, the R binding domain interacts with the N-terminal dimerization domain of R(I)α and R(II)α. A putative RGS domain was identified near the N-terminal region of D-AKAP2. The presence of this domain raises the intriguing possibility that D-AKAP2 may interact with a Gα protein thus providing a link between the signaling machinery at the plasma membrane and the downstream kinase.

Original languageEnglish (US)
Pages (from-to)11184-11189
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number21
DOIs
StatePublished - Oct 14 1997

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Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Testis
Organism Cloning
Proteins
Complementary DNA
Messenger RNA
Dimerization
GTP-Binding Proteins
Skeletal Muscle
Phosphotransferases
Cell Membrane
Kidney
Amino Acids
Brain

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain. / Huang, Lily Jun Shen; Durick, Kyle; Weiner, Joshua A.; Chun, Jerold; Taylor, Susan S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 21, 14.10.1997, p. 11184-11189.

Research output: Contribution to journalArticle

Huang, Lily Jun Shen ; Durick, Kyle ; Weiner, Joshua A. ; Chun, Jerold ; Taylor, Susan S. / D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 21. pp. 11184-11189.
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