D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices

Gemma Molinaro, Sara Pietracupa, Luisa Di Menna, Lorenzo Pescatori, Alessandro Usiello, Giuseppe Battaglia, Ferdinando Nicoletti, Valeria Bruno

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The d-amino acid, d-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than l-glutamate. The action of d-aspartate was concentration-dependent with an apparent EC50 value of 1mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by d-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that d-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.

Original languageEnglish (US)
Pages (from-to)128-130
Number of pages3
JournalNeuroscience Letters
Volume478
Issue number3
DOIs
StatePublished - Jul 1 2010

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Keywords

  • Cortical slices
  • D-Aspartate
  • Hippocampal slices
  • Metabotropic glutamate receptors
  • Polyphosphoinositide hydrolysis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Molinaro, G., Pietracupa, S., Di Menna, L., Pescatori, L., Usiello, A., Battaglia, G., Nicoletti, F., & Bruno, V. (2010). D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices. Neuroscience Letters, 478(3), 128-130. https://doi.org/10.1016/j.neulet.2010.04.077