D-box is required for the degradation of human Shugoshin and chromosome alignment

Guosheng Fu, Shasha Hua, Tarsha Ward, Xia Ding, Yong Yang, Zhen Guo, Xuebiao Yao

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Chromosome segregation and proper alignment in mitosis relies on cohesion between sister chromatids and the interaction of the kinetochore with spindle microtubules. Vertebrate Sgo1 localizes to kinetochores and is required to prevent premature sister centromere separation in mitosis. Sgo1 is degraded by the anaphase-promoting complex, allowing the separation of sister centromeres in anaphase. However, little is known about the molecular basis of Sgo1 degradation and its temporal control during mitosis. Here, we show that APC/C targets human Sgo1 for degradation through a destruction box motif (D-box) in its C-terminus. Mutation in the D-box causes transient metaphase arrest, and mutation in the D-box leads to defects in chromosome alignment and segregation through its effect on the localization of Aurora B and CENP-E. These results provide a link between sister centromere cohesion and bipolar attachment of kinetochores.

Original languageEnglish (US)
Pages (from-to)672-678
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number3
DOIs
StatePublished - Jun 8 2007

Keywords

  • Aurora B
  • Chromosome segregation
  • D-box
  • Degradation
  • Mitosis
  • Sgo

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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