Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions

Victoria A. Acosta-Rodríguez, Sebastián Márquez, Gabriela A. Salvador, Susana J. Pasquaré, Lucas D. Gorné, Eduardo Garbarino-Pico, Norma M. Giusto, Mario Eduardo Guido

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Circadian clocks regulate the temporal organization of several biochemical processes, including lipid metabolism, and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in a ) the labeling of total [ 3H] GPLs, with lowest levels around 28 and 56 h, and b ) the activity of GPL-synthesizing and GPL-remodeling enzymes, such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT), respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with choline kinase (ChoK) and CTP:phosphocholine cytidylyl-tranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes, with the lowest levels every ∼28 h, that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.

Original languageEnglish (US)
Pages (from-to)1798-1811
Number of pages14
JournalJournal of lipid research
Volume54
Issue number7
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Fingerprint

Glycerophospholipids
Fibroblasts
Phosphatidylcholines
Cell culture
Choline Kinase
Labeling
Cytidine Triphosphate
Clocks
Circadian Clocks
Phosphorylcholine
Enzymes
1-Acylglycerophosphocholine O-Acyltransferase
Biochemical Phenomena
Phosphatidate Phosphatase
NIH 3T3 Cells
Biosynthesis
Lipid Metabolism
Metabolism
Shock
Phospholipids

Keywords

  • Circadian rhythm
  • Phospholipid
  • Serum shock
  • Synchronization

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Acosta-Rodríguez, V. A., Márquez, S., Salvador, G. A., Pasquaré, S. J., Gorné, L. D., Garbarino-Pico, E., ... Guido, M. E. (2013). Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions. Journal of lipid research, 54(7), 1798-1811. https://doi.org/10.1194/jlr.M034264

Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions. / Acosta-Rodríguez, Victoria A.; Márquez, Sebastián; Salvador, Gabriela A.; Pasquaré, Susana J.; Gorné, Lucas D.; Garbarino-Pico, Eduardo; Giusto, Norma M.; Guido, Mario Eduardo.

In: Journal of lipid research, Vol. 54, No. 7, 01.07.2013, p. 1798-1811.

Research output: Contribution to journalArticle

Acosta-Rodríguez, VA, Márquez, S, Salvador, GA, Pasquaré, SJ, Gorné, LD, Garbarino-Pico, E, Giusto, NM & Guido, ME 2013, 'Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions', Journal of lipid research, vol. 54, no. 7, pp. 1798-1811. https://doi.org/10.1194/jlr.M034264
Acosta-Rodríguez, Victoria A. ; Márquez, Sebastián ; Salvador, Gabriela A. ; Pasquaré, Susana J. ; Gorné, Lucas D. ; Garbarino-Pico, Eduardo ; Giusto, Norma M. ; Guido, Mario Eduardo. / Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions. In: Journal of lipid research. 2013 ; Vol. 54, No. 7. pp. 1798-1811.
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