DAZL 260A>G and MTHFR 677C>T variants in sperm DNA of infertile

Kishlay Kumar, S. Venkatesh, P. R. Sharma, P. K. Tiwari, Rima Dada

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Abstract

DAZL (deleted in azoospermia-like) 260A>G and MTHFR (methylene tetrahydrofolate reductase) 677C>T are two important autosomal variants associated with impaired spermatogenesis. In this study, we investigated DAZL 260A>G and MTHFR 677C>T variants in sperm DNA and their frequency in oligozoospermic infertile men of Indian origin. The study on sperm DNA was performed, since it is more prone to oxidative stress-induced damage and mutation. One hundred oligozoopsermic infertile men having normal chromosomal complement with intact Y chromosome and 100 age- and ethnically-matched fertile controls were investigated for these variants in their sperm genome. Spermatozoa were separated by gradient centrifugation and DNA was isolated and analyzed for the single nucleotide polymorphisms (SNPs) by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The results showed no significant differences in the frequency of DAZL AG (P = 0.58) and MTHFR CT (P = 0.44) between oligozoospermic infertile men and controls. However, 8% (8/100) oligozoospermic infertile men harbored both the variants and showed significantly (P<0.0001) lower sperm count (3.28 ± 1.1 vs 12.50 ± 4.09) compared to infertile men with either of the single variant. None of the fertile controls showed the presence of the both variants. In conclusion, the combined effect of both DAZL 260A>G and MTHFR 677C>T variants may have role in compromised sperm count. However, further studies are required to find the pathological role of these combined variants in male infertility.

Original languageEnglish (US)
Pages (from-to)422-426
Number of pages5
JournalIndian Journal of Biochemistry and Biophysics
Volume48
Issue number6
StatePublished - Dec 1 2011

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Keywords

  • DAZL 260A>G
  • MTHFR 677C>T
  • Male infertility
  • Oligozoospermic
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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