DdrB stimulates single-stranded DNA annealing and facilitates RecA-independent DNA repair in Deinococcus radiodurans

Guangzhi Xu, Huiming Lu, Liangyan Wang, Huan Chen, Zhenjian Xu, Yihuai Hu, Bing Tian, Yuejin Hua

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The bacterium Deinococcus radiodurans can survive extremely high exposure to ionizing radiation. The repair mechanisms involved in this extraordinary ability are still being investigated. ddrB is one gene that is highly up-regulated after irradiation, and it has been proposed to be involved in RecA-independent repair in D. radiodurans. Here we cloned, expressed and characterized ddrB in order to define its roles in the radioresistance of D. radiodurans. DdrB preferentially binds to single-stranded DNA. Moreover, it interacts directly with single-stranded binding protein of D. radiodurans DrSSB, and stimulates single-stranded DNA annealing even in the presence of DrSSB. The post-irradiation DNA repair kinetics of a ddrB/recA double mutant were compared to ddrB and recA single mutants by pulsed-field gel electrophoresis (PFGE). DNA fragment rejoining in the ddrB/recA double mutant is severely compromised, suggesting that DdrB-mediated single-stranded annealing plays a critical role in the RecA-independent DNA repair of D. radiodurans.

Original languageEnglish (US)
Pages (from-to)805-812
Number of pages8
JournalDNA Repair
Volume9
Issue number7
DOIs
StatePublished - Jul 1 2010

Fingerprint

Deinococcus
Single-Stranded DNA
DNA Repair
Repair
Annealing
DNA
Irradiation
Ionizing radiation
Electrophoresis
Pulsed Field Gel Electrophoresis
Bacteria
Carrier Proteins
Ionizing Radiation
Genes
Gels
Kinetics

Keywords

  • DdrB
  • Deinococcus radiodurans
  • RecA-independent DNA repair
  • SSA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

DdrB stimulates single-stranded DNA annealing and facilitates RecA-independent DNA repair in Deinococcus radiodurans. / Xu, Guangzhi; Lu, Huiming; Wang, Liangyan; Chen, Huan; Xu, Zhenjian; Hu, Yihuai; Tian, Bing; Hua, Yuejin.

In: DNA Repair, Vol. 9, No. 7, 01.07.2010, p. 805-812.

Research output: Contribution to journalArticle

Xu, Guangzhi ; Lu, Huiming ; Wang, Liangyan ; Chen, Huan ; Xu, Zhenjian ; Hu, Yihuai ; Tian, Bing ; Hua, Yuejin. / DdrB stimulates single-stranded DNA annealing and facilitates RecA-independent DNA repair in Deinococcus radiodurans. In: DNA Repair. 2010 ; Vol. 9, No. 7. pp. 805-812.
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