De novo lumen formation and elongation in the developing nephron: A central role for afadin in apical polarity

Zhufeng Yang, Susan Zimmerman, Paul R. Brakeman, Gerard M. Beaudoin, Louis F. Reichardt, Denise K. Marciano

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A fundamental process in biology is the de novo formation and morphogenesis of polarized tubules. Although these processes are essential for the formation of multiple metazoan organ systems, little is known about the molecular mechanisms that regulate them. In this study, we have characterized several steps in tubule formation and morphogenesis using the mouse kidney as a model system. We report that kidney mesenchymal cells contain discrete Par3-expressing membrane microdomains that become restricted to an apical domain, coinciding with lumen formation. Once lumen formation has been initiated, elongation occurs by simultaneous extension and additional de novo lumen generation. We demonstrate that lumen formation and elongation require afadin, a nectin adaptor protein implicated in adherens junction formation. Mice that lack afadin in nephron precursors show evidence of Par3-expressing membrane microdomains, but fail to develop normal apical-basal polarity and generate a continuous lumen. Absence of afadin led to delayed and diminished integration of nectin complexes and failure to recruit R-cadherin. Furthermore, we demonstrate that afadin is required for Par complex formation. Together, these results suggest that afadin acts upstream of the Par complex to regulate the integration and/or coalescence of membrane microdomains, thereby establishing apical-basal polarity and lumen formation/elongation during kidney tubulogenesis.

Original languageEnglish (US)
Pages (from-to)1774-1784
Number of pages11
JournalDevelopment (Cambridge)
Volume140
Issue number8
DOIs
StatePublished - Apr 2013

Fingerprint

Nephrons
Membrane Microdomains
Kidney
Morphogenesis
Adherens Junctions
afadin
Proteins
nectins

Keywords

  • Afadin (Mllt4)
  • Cadherin
  • Kidney development
  • Lumen
  • Nectin
  • Par complex
  • Polarity
  • Tubulogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

De novo lumen formation and elongation in the developing nephron : A central role for afadin in apical polarity. / Yang, Zhufeng; Zimmerman, Susan; Brakeman, Paul R.; Beaudoin, Gerard M.; Reichardt, Louis F.; Marciano, Denise K.

In: Development (Cambridge), Vol. 140, No. 8, 04.2013, p. 1774-1784.

Research output: Contribution to journalArticle

Yang, Zhufeng ; Zimmerman, Susan ; Brakeman, Paul R. ; Beaudoin, Gerard M. ; Reichardt, Louis F. ; Marciano, Denise K. / De novo lumen formation and elongation in the developing nephron : A central role for afadin in apical polarity. In: Development (Cambridge). 2013 ; Vol. 140, No. 8. pp. 1774-1784.
@article{ae0c8ddb73b94e9b81e654c958f3b799,
title = "De novo lumen formation and elongation in the developing nephron: A central role for afadin in apical polarity",
abstract = "A fundamental process in biology is the de novo formation and morphogenesis of polarized tubules. Although these processes are essential for the formation of multiple metazoan organ systems, little is known about the molecular mechanisms that regulate them. In this study, we have characterized several steps in tubule formation and morphogenesis using the mouse kidney as a model system. We report that kidney mesenchymal cells contain discrete Par3-expressing membrane microdomains that become restricted to an apical domain, coinciding with lumen formation. Once lumen formation has been initiated, elongation occurs by simultaneous extension and additional de novo lumen generation. We demonstrate that lumen formation and elongation require afadin, a nectin adaptor protein implicated in adherens junction formation. Mice that lack afadin in nephron precursors show evidence of Par3-expressing membrane microdomains, but fail to develop normal apical-basal polarity and generate a continuous lumen. Absence of afadin led to delayed and diminished integration of nectin complexes and failure to recruit R-cadherin. Furthermore, we demonstrate that afadin is required for Par complex formation. Together, these results suggest that afadin acts upstream of the Par complex to regulate the integration and/or coalescence of membrane microdomains, thereby establishing apical-basal polarity and lumen formation/elongation during kidney tubulogenesis.",
keywords = "Afadin (Mllt4), Cadherin, Kidney development, Lumen, Nectin, Par complex, Polarity, Tubulogenesis",
author = "Zhufeng Yang and Susan Zimmerman and Brakeman, {Paul R.} and Beaudoin, {Gerard M.} and Reichardt, {Louis F.} and Marciano, {Denise K.}",
year = "2013",
month = "4",
doi = "10.1242/dev.087957",
language = "English (US)",
volume = "140",
pages = "1774--1784",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "8",

}

TY - JOUR

T1 - De novo lumen formation and elongation in the developing nephron

T2 - A central role for afadin in apical polarity

AU - Yang, Zhufeng

AU - Zimmerman, Susan

AU - Brakeman, Paul R.

AU - Beaudoin, Gerard M.

AU - Reichardt, Louis F.

AU - Marciano, Denise K.

PY - 2013/4

Y1 - 2013/4

N2 - A fundamental process in biology is the de novo formation and morphogenesis of polarized tubules. Although these processes are essential for the formation of multiple metazoan organ systems, little is known about the molecular mechanisms that regulate them. In this study, we have characterized several steps in tubule formation and morphogenesis using the mouse kidney as a model system. We report that kidney mesenchymal cells contain discrete Par3-expressing membrane microdomains that become restricted to an apical domain, coinciding with lumen formation. Once lumen formation has been initiated, elongation occurs by simultaneous extension and additional de novo lumen generation. We demonstrate that lumen formation and elongation require afadin, a nectin adaptor protein implicated in adherens junction formation. Mice that lack afadin in nephron precursors show evidence of Par3-expressing membrane microdomains, but fail to develop normal apical-basal polarity and generate a continuous lumen. Absence of afadin led to delayed and diminished integration of nectin complexes and failure to recruit R-cadherin. Furthermore, we demonstrate that afadin is required for Par complex formation. Together, these results suggest that afadin acts upstream of the Par complex to regulate the integration and/or coalescence of membrane microdomains, thereby establishing apical-basal polarity and lumen formation/elongation during kidney tubulogenesis.

AB - A fundamental process in biology is the de novo formation and morphogenesis of polarized tubules. Although these processes are essential for the formation of multiple metazoan organ systems, little is known about the molecular mechanisms that regulate them. In this study, we have characterized several steps in tubule formation and morphogenesis using the mouse kidney as a model system. We report that kidney mesenchymal cells contain discrete Par3-expressing membrane microdomains that become restricted to an apical domain, coinciding with lumen formation. Once lumen formation has been initiated, elongation occurs by simultaneous extension and additional de novo lumen generation. We demonstrate that lumen formation and elongation require afadin, a nectin adaptor protein implicated in adherens junction formation. Mice that lack afadin in nephron precursors show evidence of Par3-expressing membrane microdomains, but fail to develop normal apical-basal polarity and generate a continuous lumen. Absence of afadin led to delayed and diminished integration of nectin complexes and failure to recruit R-cadherin. Furthermore, we demonstrate that afadin is required for Par complex formation. Together, these results suggest that afadin acts upstream of the Par complex to regulate the integration and/or coalescence of membrane microdomains, thereby establishing apical-basal polarity and lumen formation/elongation during kidney tubulogenesis.

KW - Afadin (Mllt4)

KW - Cadherin

KW - Kidney development

KW - Lumen

KW - Nectin

KW - Par complex

KW - Polarity

KW - Tubulogenesis

UR - http://www.scopus.com/inward/record.url?scp=84875466076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875466076&partnerID=8YFLogxK

U2 - 10.1242/dev.087957

DO - 10.1242/dev.087957

M3 - Article

C2 - 23487309

AN - SCOPUS:84875466076

VL - 140

SP - 1774

EP - 1784

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 8

ER -