De novo synthesis of cholesterol by the human fetal adrenal gland

B. R. Carr, E. R. Simpson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The rate of de novo synthesis of cholesterol by human fetal adrenal tissue was computed from the rate of incorporation of tritium from [3H]water into cholesterol. Cholesterol biosynthesis in this tissue was stimulated by ACTH, as evidenced by the incorporation of tritium from [3H]water into cholesterol, the incorporation of [14C]acetate into cholesterol, and the specific activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase in microsome-enriched preparations from fetal adrenal tissue. ACTH also stimulated the incorporation of tritium from [3H]water into triglycerides but not the incorporation of [14C]oleic acid into triglycerides. When the specific activity of HMG CoA reductase was determined in microsomes prepared from tissue homogenized in the presence of NaF (50 mM), the activity was 0.3-0.6 times the specific activity in microsomes prepared from tissue homogenized in the absence of NaF, regardless of whether the tissue was fresh or had been maintained to organ culture for 3 days in the presence or absence of ACTH. The specific activity of HMG CoA reductase that was most consistent with the estimated rate of cholesterol biosynthesis, determined using the technique of incorporation of tritium from [3H]water into cholesterol, was that observed in microsomes prepared from tissue homogenized in the presence of NaF. Based on these results, we suggest that much of the HMG CoA reductase in the human fetal adrenal gland may be in an inactive form, whether or not the tissue is stimulated by ACTH. Based on a comparison of the rate of de novo synthesis of cholesterol with the rates of secretion of the steroid hormones, dehydroisoandrosterone sulfate and cortisol, it is concluded that de novo cholesterol synthesis could account for the synthesis of some 30% of the daily secretion rate of these steroids.

Original languageEnglish (US)
Pages (from-to)2154-2162
Number of pages9
JournalEndocrinology
Volume108
Issue number6
StatePublished - 1981

Fingerprint

Adrenal Glands
Cholesterol
Tritium
Microsomes
Adrenocorticotropic Hormone
Oxidoreductases
Coenzyme A
Water
Triglycerides
Fetus
Steroids
Dehydroepiandrosterone Sulfate
Organ Culture Techniques
Oleic Acid
Hydrocortisone
Hormones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

De novo synthesis of cholesterol by the human fetal adrenal gland. / Carr, B. R.; Simpson, E. R.

In: Endocrinology, Vol. 108, No. 6, 1981, p. 2154-2162.

Research output: Contribution to journalArticle

Carr, BR & Simpson, ER 1981, 'De novo synthesis of cholesterol by the human fetal adrenal gland', Endocrinology, vol. 108, no. 6, pp. 2154-2162.
Carr, B. R. ; Simpson, E. R. / De novo synthesis of cholesterol by the human fetal adrenal gland. In: Endocrinology. 1981 ; Vol. 108, No. 6. pp. 2154-2162.
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