Decreased vulnerability of hippocampal neurons after neonatal hypoxia-ischemia in bis-deficient mice

Kyung Ok Cho, Kyung Eon Lee, Dong Ye Youn, Kyoung Hoon Jeong, Joo Youn Kim, Hye Hyeon Yoon, Jeong Hwa Lee, Seong Yun Kim

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The Bcl-2-interacting death suppressor (Bis) protein is involved in antiapoptosis and antistress pathways. However, its roles after neonatal hypoxia-ischemia remain obscure. Therefore, we investigated the effects of Bis deletion on hippocampal cell death following neonatal hypoxia-ischemia. We transected the right common carotid artery of bis+/+ and bis-/- mice at postnatal Day 7 and subjected them to hypoxia for 35 min. Cresyl violet staining showed that hypoxia-ischemia induced progressive cell death in the hippocampi of bis+/+ mice. Moreover, Bis was expressed in astrocytes, not microglia, in sham-manipulated hippocampi of bis+/+ mice, and was markedly enhanced after hypoxia-ischemia. Immunoblotting showed that Bis expression significantly increased 3 and 7 days following hypoxia-ischemia. Unexpectedly, 7 days after hypoxia-ischemia, the number of hippocampal NeuN-positive cells was higher in the bis-/- mice than in the bis+/+ mice. We subsequently performed transcriptomic analysis and quantitative real time polymerase chain reaction to search for the underlying genes responsible for resistance to hypoxia-ischemia in the bis-/- hippocampus. These studies showed that 6 h after hypoxia-ischemia, galectin 3 and filamin C levels increased to a lesser extent in the bis-/- hippocampi compared with the bis+/+ hippocampi. Finally, our in vitro hypoxia-ischemia model, using A172 glioma cells and primary astrocytes, showed that downregulation of Bis blocked the enhanced expression of galectin 3 after oxygen-glucose deprivation. This study demonstrated that Bis was upregulated in the astrocytes after hypoxia-ischemia. In addition, we showed that hippocampal neurons are less vulnerable to hypoxia-ischemia in mice lacking Bis, possibly because of the modulation of galectin 3 induction.

Original languageEnglish (US)
Pages (from-to)1915-1929
Number of pages15
JournalGLIA
Volume60
Issue number12
DOIs
StatePublished - Dec 1 2012

Keywords

  • Astrocytes
  • Bis (Bag3)
  • Brain
  • Galectin 3
  • Hippocampus
  • Neonatal hypoxia-ischemia

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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    Cho, K. O., Lee, K. E., Youn, D. Y., Jeong, K. H., Kim, J. Y., Yoon, H. H., Lee, J. H., & Kim, S. Y. (2012). Decreased vulnerability of hippocampal neurons after neonatal hypoxia-ischemia in bis-deficient mice. GLIA, 60(12), 1915-1929. https://doi.org/10.1002/glia.22407