Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines

Neda S T Hozhabri, M. Douglas Benson, Michael D. Vu, Rinkesh H. Patel, Rebecca M. Martinez, Fatemeh N. Nakhaie, Harry K W Kim, Venu G. Varanasi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Inflammatory response in the dental pulp can alter the collagen matrix formation by dental pulp stem cells and lead to a delay or poor healing of the pulp. This inflammatory response is mediated by cytokines, including interleukin-1β and tumor necrosis factor-α. In this study, it is hypothesized that suppressing the actions of these inflammatory cytokines by knocking down the activity of transcription factor Nuclear Factor-κB will lead to dental pulp stem cell differentiation into odontoblasts and the production of collagen. Here, the role of Nuclear Factor-κB signaling and its reduction was examined during odontogenic behavior in the presence of these cytokines. The results showed a significant increase in Nuclear Factor-κB gene expression and p65 protein expression by interleukin-1β and tumor necrosis factor-α. Nuclear Factor-κB activation in the presence of these cytokines decreased significantly in a dose-dependent manner by a Nuclear Factor-κB inhibitor (MG132) and p65 siRNA. Down-regulation of Nuclear Factor-κB activity also enhanced the gene expression of the odontoblastic markers (dentin sialophosphoprotein, Nestin, and alkaline phosphatase) and displayed an odontoblastic cell morphology indicating the promotion of odontogenic differentiation of dental pulp stem cells. Finally, dental pulp stem cells exposed to reduced Nuclear Factor-κB activity resulted in a significant increase in collagen (I)-α1 expression in the presence of these cytokines. In conclusion, a decrease in Nuclear Factor-κB in dental pulp stem cells in the presence of inflammatory cytokines enhanced odontoblastic differentiation and collagen matrix formation.

Original languageEnglish (US)
Article numbere0113334
JournalPLoS One
Volume10
Issue number1
DOIs
StatePublished - Jan 28 2015

Fingerprint

tooth pulp
Dental Pulp
Stem cells
Pulp
stem cells
collagen
cytokines
Collagen
Stem Cells
Cytokines
tumor necrosis factors
interleukin-1
Interleukin-1
Gene expression
Tumor Necrosis Factor-alpha
inflammation
Odontoblasts
Gene Expression
Nestin
gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hozhabri, N. S. T., Benson, M. D., Vu, M. D., Patel, R. H., Martinez, R. M., Nakhaie, F. N., ... Varanasi, V. G. (2015). Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines. PLoS One, 10(1), [e0113334]. https://doi.org/10.1371/journal.pone.0113334

Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines. / Hozhabri, Neda S T; Benson, M. Douglas; Vu, Michael D.; Patel, Rinkesh H.; Martinez, Rebecca M.; Nakhaie, Fatemeh N.; Kim, Harry K W; Varanasi, Venu G.

In: PLoS One, Vol. 10, No. 1, e0113334, 28.01.2015.

Research output: Contribution to journalArticle

Hozhabri, Neda S T ; Benson, M. Douglas ; Vu, Michael D. ; Patel, Rinkesh H. ; Martinez, Rebecca M. ; Nakhaie, Fatemeh N. ; Kim, Harry K W ; Varanasi, Venu G. / Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines. In: PLoS One. 2015 ; Vol. 10, No. 1.
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