Defective internalization of low density lipoprotein in epidermoid cervical cancer cells

D. Gal, E. R. Simpson, J. C. Porter, J. M. Snyder

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cells of an epidermoid cancer cell line of human uterine cervix, which possessed a high-affinity, specific receptor for low density lipoprotein (LDL), internalized and degraded [125I]iodo-LDL at a very low rate. In these cells, LDL did not stimulate cholesteryl ester synthesis, nor did it suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase to the same extent as in the control cells. The binding of [125I]iodo-LDL by these cells was not decreased by preincubation of the cells in medium containing LDL. Using ferritin-labeled LDL (F-LDL) and electron microscopy, it was determined that at 4°C the cells bound F-LDL in the same way as other cancer cell lines that did not have a defect in internalization. When these cells were warmed to 37°C the F-LDL remained on the surface, whereas in cells from control cancer cell lines the F-LDL was internalized and was no longer observed on the cell surface. On the basis of the results of these studies it is concluded that cells of this epidermoid cancer cell line have a defective ability to internalize LDL.

Original languageEnglish (US)
Pages (from-to)597-603
Number of pages7
JournalJournal of Cell Biology
Volume92
Issue number3
StatePublished - 1982

Fingerprint

LDL Lipoproteins
Uterine Cervical Neoplasms
Ferritins
Cell Line
Neoplasms
Cholesterol Esters
LDL Receptors
Cervix Uteri
Electron Microscopy
Oxidoreductases
Cell Count

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gal, D., Simpson, E. R., Porter, J. C., & Snyder, J. M. (1982). Defective internalization of low density lipoprotein in epidermoid cervical cancer cells. Journal of Cell Biology, 92(3), 597-603.

Defective internalization of low density lipoprotein in epidermoid cervical cancer cells. / Gal, D.; Simpson, E. R.; Porter, J. C.; Snyder, J. M.

In: Journal of Cell Biology, Vol. 92, No. 3, 1982, p. 597-603.

Research output: Contribution to journalArticle

Gal, D, Simpson, ER, Porter, JC & Snyder, JM 1982, 'Defective internalization of low density lipoprotein in epidermoid cervical cancer cells', Journal of Cell Biology, vol. 92, no. 3, pp. 597-603.
Gal, D. ; Simpson, E. R. ; Porter, J. C. ; Snyder, J. M. / Defective internalization of low density lipoprotein in epidermoid cervical cancer cells. In: Journal of Cell Biology. 1982 ; Vol. 92, No. 3. pp. 597-603.
@article{51f8529a9d004788b637ce17e821e8bc,
title = "Defective internalization of low density lipoprotein in epidermoid cervical cancer cells",
abstract = "Cells of an epidermoid cancer cell line of human uterine cervix, which possessed a high-affinity, specific receptor for low density lipoprotein (LDL), internalized and degraded [125I]iodo-LDL at a very low rate. In these cells, LDL did not stimulate cholesteryl ester synthesis, nor did it suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase to the same extent as in the control cells. The binding of [125I]iodo-LDL by these cells was not decreased by preincubation of the cells in medium containing LDL. Using ferritin-labeled LDL (F-LDL) and electron microscopy, it was determined that at 4°C the cells bound F-LDL in the same way as other cancer cell lines that did not have a defect in internalization. When these cells were warmed to 37°C the F-LDL remained on the surface, whereas in cells from control cancer cell lines the F-LDL was internalized and was no longer observed on the cell surface. On the basis of the results of these studies it is concluded that cells of this epidermoid cancer cell line have a defective ability to internalize LDL.",
author = "D. Gal and Simpson, {E. R.} and Porter, {J. C.} and Snyder, {J. M.}",
year = "1982",
language = "English (US)",
volume = "92",
pages = "597--603",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - Defective internalization of low density lipoprotein in epidermoid cervical cancer cells

AU - Gal, D.

AU - Simpson, E. R.

AU - Porter, J. C.

AU - Snyder, J. M.

PY - 1982

Y1 - 1982

N2 - Cells of an epidermoid cancer cell line of human uterine cervix, which possessed a high-affinity, specific receptor for low density lipoprotein (LDL), internalized and degraded [125I]iodo-LDL at a very low rate. In these cells, LDL did not stimulate cholesteryl ester synthesis, nor did it suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase to the same extent as in the control cells. The binding of [125I]iodo-LDL by these cells was not decreased by preincubation of the cells in medium containing LDL. Using ferritin-labeled LDL (F-LDL) and electron microscopy, it was determined that at 4°C the cells bound F-LDL in the same way as other cancer cell lines that did not have a defect in internalization. When these cells were warmed to 37°C the F-LDL remained on the surface, whereas in cells from control cancer cell lines the F-LDL was internalized and was no longer observed on the cell surface. On the basis of the results of these studies it is concluded that cells of this epidermoid cancer cell line have a defective ability to internalize LDL.

AB - Cells of an epidermoid cancer cell line of human uterine cervix, which possessed a high-affinity, specific receptor for low density lipoprotein (LDL), internalized and degraded [125I]iodo-LDL at a very low rate. In these cells, LDL did not stimulate cholesteryl ester synthesis, nor did it suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase to the same extent as in the control cells. The binding of [125I]iodo-LDL by these cells was not decreased by preincubation of the cells in medium containing LDL. Using ferritin-labeled LDL (F-LDL) and electron microscopy, it was determined that at 4°C the cells bound F-LDL in the same way as other cancer cell lines that did not have a defect in internalization. When these cells were warmed to 37°C the F-LDL remained on the surface, whereas in cells from control cancer cell lines the F-LDL was internalized and was no longer observed on the cell surface. On the basis of the results of these studies it is concluded that cells of this epidermoid cancer cell line have a defective ability to internalize LDL.

UR - http://www.scopus.com/inward/record.url?scp=0020079844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020079844&partnerID=8YFLogxK

M3 - Article

C2 - 6282889

AN - SCOPUS:0020079844

VL - 92

SP - 597

EP - 603

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

ER -