Deficiency of a novel mismatch repair activity in a bladder tumor cell line

Liya Gu, Jianzin Wu, Bei Bei Zhu, Guo Min Li

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

We demonstrate here that a cell line derived from a bladder cancer is defective in strand-specific mismatch repair. The mismatch repair deficiency in this cell line is associated with microsatellite instability and blocks an early step in the repair pathway. Since the addition of a known mismatch repair component hMutSα, hMutSβ, hMutLα, replication protein A or proliferating cellular nuclear antigen could not restore mismatch repair to the mutant extract, the bladder tumor cell line is likely to be defective in an uncharacterized repair component. However, the repair in the mutant extract could be complemented by a partially purified activity derived from HeLa nuclear extracts. Therefore, in addition to revealing that a loss of mismatch repair function is associated with bladder cancer, this study provides information implicating a new mismatch repair activity.

Original languageEnglish (US)
Pages (from-to)2758-2763
Number of pages6
JournalNucleic acids research
Volume30
Issue number13
DOIs
StatePublished - Jul 1 2002

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ASJC Scopus subject areas

  • Genetics

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