Deficits in serum amyloid A contribute to increased neonatal mortality during murine listeriosis

J. Seth Hawkins, QingQing Wu, Yanxia Wang, Christopher Y. Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: To understand the increased susceptibility of preterm neonates to infection. Methods: A murine listeriosis model using immunohistochemistry, microarray technology, and real-time polymerase chain reaction (PCR). Results: We report that recombinant serum amyloid A (SAA) administered prophylactically 18 h before intraperitoneal (i.p.) inoculation with Listeria monocytogenes conferred a dramatic survival benefit compared with administration of only vehicle in neonatal mice. Neonates that received the recombinant SAA protein had significantly fewer Listeria colony counts on plating of infected liver and showed significantly more activated macrophages, but SAA did not affect postnatal growth. Real-time PCR was used to confirm the microarray findings that gene expression levels for the SAA proteins 1 (Saa1) and 2 (Saa2), in addition to that for orosomucoid-2 (Orm2), were strikingly elevated in the adult compared with those in the neonate. Real-time PCR analysis showed that of the acute phase cytokines, tumor necrosis factor (TNF) gene expression increased exponentially with time in the infected adult, whereas neonates did not show similar increases. Conclusion: The increased susceptibility of neonatal mice to listeriosis is in part mediated by a deficiency in the acute phase response, specifically expression of SAA, and that prophylactic SAA protein before neonatal murine listeriosis results in more macrophage activation, lower Listeria counts, and greater survival.

Original languageEnglish (US)
Pages (from-to)668-674
Number of pages7
JournalPediatric Research
Volume74
Issue number6
DOIs
StatePublished - Dec 2013

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Serum Amyloid A Protein
Listeriosis
Infant Mortality
Real-Time Polymerase Chain Reaction
Listeria
Orosomucoid
Gene Expression
Acute-Phase Reaction
Macrophage Activation
Listeria monocytogenes
Tumor Necrosis Factor-alpha
Immunohistochemistry
Macrophages
Cytokines
Technology
Liver
Growth
Infection

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Deficits in serum amyloid A contribute to increased neonatal mortality during murine listeriosis. / Seth Hawkins, J.; Wu, QingQing; Wang, Yanxia; Lu, Christopher Y.

In: Pediatric Research, Vol. 74, No. 6, 12.2013, p. 668-674.

Research output: Contribution to journalArticle

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