Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells

María Sánchez-Aragó, Javier García-Bermúdez, Inmaculada Martínez-Reyes, Fulvio Santacatterina, José M. Cuezva

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state.

Original languageEnglish (US)
Pages (from-to)638-644
Number of pages7
JournalEMBO Reports
Volume14
Issue number7
DOIs
StatePublished - Jun 2013
Externally publishedYes

Keywords

  • ATPase inhibitory factor 1
  • H-ATP synthase
  • cellular differentiation
  • mitochondria
  • protein degradation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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