Degradation of the framework of the Chlamydomonas cell wall by proteases present in a commercially available alpha-amylase preparation.

S. H. Imam, W. J. Snell

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A commercially available alpha-amylase derived from Bacillus licheniformis contained an enzymatic activity able to degrade the inner portion or framework of the cell wall of Chlamydomonas reinhardtii. Both the wall-degrading activity and the contaminating protease were destroyed by heating the alpha-amylase preparation at 90 degrees C for 30 min. Since the alpha-amylase activity was uneffected by heat treatment, we conclude that it was not the alpha-amylase but the contaminating protease in the preparation that was responsible for the cell wall-degrading activity.

Original languageEnglish (US)
Pages (from-to)1701-1704
Number of pages4
JournalApplied and environmental microbiology
Volume53
Issue number7
DOIs
StatePublished - Jul 1987

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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