Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress

Eui Man Jeong; Chai Wan Kim; Sung Yup Cho; Gi Yong Jang; Dong Myung Shin; Ju Hong Jeon; In Gyu Kim

doi:10.1016/j.febslet.2009.01.032

Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress

Eui Man Jeong, Chai Wan Kim, Sung Yup Cho, Gi Yong Jang, Dong Myung Shin, Ju Hong Jeon, In Gyu Kim

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There is conflicting evidence on the role of TG2 in apoptosis. In this report, we show that TG2 increases in response to low level of oxidative stress, whereas TG2 diminishes under high stress conditions. Monitoring TG2 expression, activity and calcium concentration in cells treated with A23187 revealed that the initial rise of calcium activates TG2 but subsequent calcium-overload induces the degradation of TG2 via calcium-mediated polyubiquitination. These results indicate that the role of TG2 in apoptosis depends on the level of calcium influx triggered by oxidative stress. Structured summary: MINT-6824687: TG2 (uniprotkb:P21980) physically interacts (MI:0218) with Ubiquitin (uniprotkb:P62988) by anti bait coimmunoprecipitation (MI:0006).

Original language	English (US)
Pages (from-to)	648-654
Number of pages	7
Journal	FEBS Letters
Volume	583
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2009.01.032
State	Published - Feb 18 2009

Keywords

Calcium-overload
Oxidative stress
Proteasomal degradation
Transglutaminase 2
Ubiquitination

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.01.032

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title = "Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress",

abstract = "Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There is conflicting evidence on the role of TG2 in apoptosis. In this report, we show that TG2 increases in response to low level of oxidative stress, whereas TG2 diminishes under high stress conditions. Monitoring TG2 expression, activity and calcium concentration in cells treated with A23187 revealed that the initial rise of calcium activates TG2 but subsequent calcium-overload induces the degradation of TG2 via calcium-mediated polyubiquitination. These results indicate that the role of TG2 in apoptosis depends on the level of calcium influx triggered by oxidative stress. Structured summary: MINT-6824687: TG2 (uniprotkb:P21980) physically interacts (MI:0218) with Ubiquitin (uniprotkb:P62988) by anti bait coimmunoprecipitation (MI:0006).",

keywords = "Calcium-overload, Oxidative stress, Proteasomal degradation, Transglutaminase 2, Ubiquitination",

author = "Jeong, {Eui Man} and Kim, {Chai Wan} and Cho, {Sung Yup} and Jang, {Gi Yong} and Shin, {Dong Myung} and Jeon, {Ju Hong} and Kim, {In Gyu}",

note = "Funding Information: We thank Dr. Y.D. Kim for critical comments on the manuscript and Dr. Dirk Bohmann (University of Rochester Medical Center, Rochester, NY 14642, USA) for kindly providing the pMT123HA-ubiquitin construct. This work was supported by the grants from Korea Science and Engineering Foundation (R11-2002-097-09005-0 and R01-2005-000-10364-0) and also by the Research Program for New Drug Target Discovery (M10748000296-07N4800-29610). S.Y.C., E.M.J., and G.Y.J. were supported by the graduate program of BK21, Korean Ministry of Education, Science and Technology.",

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journal = "FEBS Letters",

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T1 - Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress

AU - Jeong, Eui Man

AU - Kim, Chai Wan

AU - Cho, Sung Yup

AU - Jang, Gi Yong

AU - Shin, Dong Myung

AU - Jeon, Ju Hong

AU - Kim, In Gyu

N1 - Funding Information: We thank Dr. Y.D. Kim for critical comments on the manuscript and Dr. Dirk Bohmann (University of Rochester Medical Center, Rochester, NY 14642, USA) for kindly providing the pMT123HA-ubiquitin construct. This work was supported by the grants from Korea Science and Engineering Foundation (R11-2002-097-09005-0 and R01-2005-000-10364-0) and also by the Research Program for New Drug Target Discovery (M10748000296-07N4800-29610). S.Y.C., E.M.J., and G.Y.J. were supported by the graduate program of BK21, Korean Ministry of Education, Science and Technology.

PY - 2009/2/18

Y1 - 2009/2/18

N2 - Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There is conflicting evidence on the role of TG2 in apoptosis. In this report, we show that TG2 increases in response to low level of oxidative stress, whereas TG2 diminishes under high stress conditions. Monitoring TG2 expression, activity and calcium concentration in cells treated with A23187 revealed that the initial rise of calcium activates TG2 but subsequent calcium-overload induces the degradation of TG2 via calcium-mediated polyubiquitination. These results indicate that the role of TG2 in apoptosis depends on the level of calcium influx triggered by oxidative stress. Structured summary: MINT-6824687: TG2 (uniprotkb:P21980) physically interacts (MI:0218) with Ubiquitin (uniprotkb:P62988) by anti bait coimmunoprecipitation (MI:0006).

AB - Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There is conflicting evidence on the role of TG2 in apoptosis. In this report, we show that TG2 increases in response to low level of oxidative stress, whereas TG2 diminishes under high stress conditions. Monitoring TG2 expression, activity and calcium concentration in cells treated with A23187 revealed that the initial rise of calcium activates TG2 but subsequent calcium-overload induces the degradation of TG2 via calcium-mediated polyubiquitination. These results indicate that the role of TG2 in apoptosis depends on the level of calcium influx triggered by oxidative stress. Structured summary: MINT-6824687: TG2 (uniprotkb:P21980) physically interacts (MI:0218) with Ubiquitin (uniprotkb:P62988) by anti bait coimmunoprecipitation (MI:0006).

KW - Calcium-overload

KW - Oxidative stress

KW - Proteasomal degradation

KW - Transglutaminase 2

KW - Ubiquitination

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Degradation of transglutaminase 2 by calcium-mediated ubiquitination responding to high oxidative stress

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