Deletion mutants which affect the nuclease-sensitive site in simian virus 40 chromatin

R. D. Gerard, M. Woodworth-Gutai, W. A. Scott

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A short segment of simian virus 40 (SV 40) chromatin on the late side of the origin of replication is hypersensitive to nuclease cleavage. The role of DNA sequence information in this nuclease-sensitive feature was examined by constructing segment of in(Or)-1411 (a viable, partially duplicated variant of SV 40), and nuclease sensitivity of the inserted segment was compared with that of the unaltered sequences in their normal location in the viral genome. Extended deletions (118 to 161 base pairs) essentially abolished nuclease sensitivity within the inserted segment. Shorter deletions (21 to 52 base pairs) at separate locations retained the nuclease-sensitive feature. In some short-deletion mutants nuclease susceptibility was substantially reduced. We conclude that more than one genetic element in this region contributes to the organization of the nuclease-sensitive feature and that the SV 40 72-base repeat is not, in itself, sufficient signal for this feature.

Original languageEnglish (US)
Pages (from-to)782-788
Number of pages7
JournalMolecular and cellular biology
Volume2
Issue number7
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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