Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2

Srinivas Malladi, Kishore V L Parsa, Deepthi Bhupathi, María A. Rodríguez-González, Juan A. Conde, Pallavi Anumula, Hannah E. Romo, Cheryl J. Claunch, Rafael P. Ballestero, Maribel González-García

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bcl-2 is an anti-apoptotic member of the Bcl-2 family of proteins that protects cells from apoptosis induced by a large variety of stimuli. The protein BMRP (MRPL41) was identified as a Bcl-2 binding partner and shown to have pro-apoptotic activity. We have performed deletion mutational analyses to identify the domain(s) of Bcl-2 and BMRP that are involved in the Bcl-2/BMRP interaction, and the region(s) of BMRP that mediate its pro-apoptotic activity. The results of these studies indicate that both the BH4 domain of Bcl-2 and its central region encompassing its BH1, BH2, and BH3 domains are required for its interaction with BMRP. The loop region and the transmembrane domain of Bcl-2 were found to be dispensable for this interaction. The Bcl-2 deletion mutants that do not interact with BMRP were previously shown to be functionally inactive. Deletion analyses of the BMRP protein delimited the region of BMRP needed for its interaction with Bcl-2 to the amino-terminal two-thirds of the protein (amino acid residues 1-92). Further deletions at either end of the BMRP(1-92) truncated protein resulted in lack of binding to Bcl-2. Functional studies performed with BMRP deletion mutants suggest that the cell death-inducing domains of the protein reside mainly within its amino-terminal two-thirds. The region of BMRP required for the interaction with Bcl-2 is very relevant for the cell death-inducing activity of the protein, suggesting that one possible mechanism by which BMRP induces cell death is by binding to and blocking the anti-apoptotic activity of Bcl-2.

Original languageEnglish (US)
Pages (from-to)217-232
Number of pages16
JournalMolecular and Cellular Biochemistry
Volume351
Issue number1-2
DOIs
StatePublished - May 1 2011

Fingerprint

Apoptosis Regulatory Proteins
Cell death
Proteins
Cell Death
Apoptosis
Amino Acids

Keywords

  • Apoptosis
  • Bcl-2
  • BMRP
  • Mitochondria
  • Ribosome

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Malladi, S., Parsa, K. V. L., Bhupathi, D., Rodríguez-González, M. A., Conde, J. A., Anumula, P., ... González-García, M. (2011). Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2. Molecular and Cellular Biochemistry, 351(1-2), 217-232. https://doi.org/10.1007/s11010-011-0729-1

Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2. / Malladi, Srinivas; Parsa, Kishore V L; Bhupathi, Deepthi; Rodríguez-González, María A.; Conde, Juan A.; Anumula, Pallavi; Romo, Hannah E.; Claunch, Cheryl J.; Ballestero, Rafael P.; González-García, Maribel.

In: Molecular and Cellular Biochemistry, Vol. 351, No. 1-2, 01.05.2011, p. 217-232.

Research output: Contribution to journalArticle

Malladi, S, Parsa, KVL, Bhupathi, D, Rodríguez-González, MA, Conde, JA, Anumula, P, Romo, HE, Claunch, CJ, Ballestero, RP & González-García, M 2011, 'Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2', Molecular and Cellular Biochemistry, vol. 351, no. 1-2, pp. 217-232. https://doi.org/10.1007/s11010-011-0729-1
Malladi, Srinivas ; Parsa, Kishore V L ; Bhupathi, Deepthi ; Rodríguez-González, María A. ; Conde, Juan A. ; Anumula, Pallavi ; Romo, Hannah E. ; Claunch, Cheryl J. ; Ballestero, Rafael P. ; González-García, Maribel. / Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2. In: Molecular and Cellular Biochemistry. 2011 ; Vol. 351, No. 1-2. pp. 217-232.
@article{417d0c4294eb4aba924050e0e161fb6a,
title = "Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2",
abstract = "Bcl-2 is an anti-apoptotic member of the Bcl-2 family of proteins that protects cells from apoptosis induced by a large variety of stimuli. The protein BMRP (MRPL41) was identified as a Bcl-2 binding partner and shown to have pro-apoptotic activity. We have performed deletion mutational analyses to identify the domain(s) of Bcl-2 and BMRP that are involved in the Bcl-2/BMRP interaction, and the region(s) of BMRP that mediate its pro-apoptotic activity. The results of these studies indicate that both the BH4 domain of Bcl-2 and its central region encompassing its BH1, BH2, and BH3 domains are required for its interaction with BMRP. The loop region and the transmembrane domain of Bcl-2 were found to be dispensable for this interaction. The Bcl-2 deletion mutants that do not interact with BMRP were previously shown to be functionally inactive. Deletion analyses of the BMRP protein delimited the region of BMRP needed for its interaction with Bcl-2 to the amino-terminal two-thirds of the protein (amino acid residues 1-92). Further deletions at either end of the BMRP(1-92) truncated protein resulted in lack of binding to Bcl-2. Functional studies performed with BMRP deletion mutants suggest that the cell death-inducing domains of the protein reside mainly within its amino-terminal two-thirds. The region of BMRP required for the interaction with Bcl-2 is very relevant for the cell death-inducing activity of the protein, suggesting that one possible mechanism by which BMRP induces cell death is by binding to and blocking the anti-apoptotic activity of Bcl-2.",
keywords = "Apoptosis, Bcl-2, BMRP, Mitochondria, Ribosome",
author = "Srinivas Malladi and Parsa, {Kishore V L} and Deepthi Bhupathi and Rodr{\'i}guez-Gonz{\'a}lez, {Mar{\'i}a A.} and Conde, {Juan A.} and Pallavi Anumula and Romo, {Hannah E.} and Claunch, {Cheryl J.} and Ballestero, {Rafael P.} and Maribel Gonz{\'a}lez-Garc{\'i}a",
year = "2011",
month = "5",
day = "1",
doi = "10.1007/s11010-011-0729-1",
language = "English (US)",
volume = "351",
pages = "217--232",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Deletion mutational analysis of BMRP, a pro-apoptotic protein that binds to Bcl-2

AU - Malladi, Srinivas

AU - Parsa, Kishore V L

AU - Bhupathi, Deepthi

AU - Rodríguez-González, María A.

AU - Conde, Juan A.

AU - Anumula, Pallavi

AU - Romo, Hannah E.

AU - Claunch, Cheryl J.

AU - Ballestero, Rafael P.

AU - González-García, Maribel

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Bcl-2 is an anti-apoptotic member of the Bcl-2 family of proteins that protects cells from apoptosis induced by a large variety of stimuli. The protein BMRP (MRPL41) was identified as a Bcl-2 binding partner and shown to have pro-apoptotic activity. We have performed deletion mutational analyses to identify the domain(s) of Bcl-2 and BMRP that are involved in the Bcl-2/BMRP interaction, and the region(s) of BMRP that mediate its pro-apoptotic activity. The results of these studies indicate that both the BH4 domain of Bcl-2 and its central region encompassing its BH1, BH2, and BH3 domains are required for its interaction with BMRP. The loop region and the transmembrane domain of Bcl-2 were found to be dispensable for this interaction. The Bcl-2 deletion mutants that do not interact with BMRP were previously shown to be functionally inactive. Deletion analyses of the BMRP protein delimited the region of BMRP needed for its interaction with Bcl-2 to the amino-terminal two-thirds of the protein (amino acid residues 1-92). Further deletions at either end of the BMRP(1-92) truncated protein resulted in lack of binding to Bcl-2. Functional studies performed with BMRP deletion mutants suggest that the cell death-inducing domains of the protein reside mainly within its amino-terminal two-thirds. The region of BMRP required for the interaction with Bcl-2 is very relevant for the cell death-inducing activity of the protein, suggesting that one possible mechanism by which BMRP induces cell death is by binding to and blocking the anti-apoptotic activity of Bcl-2.

AB - Bcl-2 is an anti-apoptotic member of the Bcl-2 family of proteins that protects cells from apoptosis induced by a large variety of stimuli. The protein BMRP (MRPL41) was identified as a Bcl-2 binding partner and shown to have pro-apoptotic activity. We have performed deletion mutational analyses to identify the domain(s) of Bcl-2 and BMRP that are involved in the Bcl-2/BMRP interaction, and the region(s) of BMRP that mediate its pro-apoptotic activity. The results of these studies indicate that both the BH4 domain of Bcl-2 and its central region encompassing its BH1, BH2, and BH3 domains are required for its interaction with BMRP. The loop region and the transmembrane domain of Bcl-2 were found to be dispensable for this interaction. The Bcl-2 deletion mutants that do not interact with BMRP were previously shown to be functionally inactive. Deletion analyses of the BMRP protein delimited the region of BMRP needed for its interaction with Bcl-2 to the amino-terminal two-thirds of the protein (amino acid residues 1-92). Further deletions at either end of the BMRP(1-92) truncated protein resulted in lack of binding to Bcl-2. Functional studies performed with BMRP deletion mutants suggest that the cell death-inducing domains of the protein reside mainly within its amino-terminal two-thirds. The region of BMRP required for the interaction with Bcl-2 is very relevant for the cell death-inducing activity of the protein, suggesting that one possible mechanism by which BMRP induces cell death is by binding to and blocking the anti-apoptotic activity of Bcl-2.

KW - Apoptosis

KW - Bcl-2

KW - BMRP

KW - Mitochondria

KW - Ribosome

UR - http://www.scopus.com/inward/record.url?scp=79953694462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953694462&partnerID=8YFLogxK

U2 - 10.1007/s11010-011-0729-1

DO - 10.1007/s11010-011-0729-1

M3 - Article

C2 - 21253851

AN - SCOPUS:79953694462

VL - 351

SP - 217

EP - 232

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -