Deletion of stably integrated DNA is suppressed by cadmium and zinc

J. M. Abrams, E. T. Schimke

Research output: Contribution to journalArticle

Abstract

The bacterial xanthine-guanine phosphoribosyltransferase (GPT) gene was fused to a metal-responsive promoter and transfected into a murine cell line. Clonal transformants harboring metal-responsive or nonresponsive GPT genes (using a thymidine kinase promoter) were then studied for the loss of transfected gene function either during periods of constitutive expression or during periods of induced activity. Nontoxic levels of cadmium and zinc markedly reduced the frequency of mutagenesis in all transfected lines irrespective of transcriptional status. A survey of 17 GPT- clones derived from two original transfectants showed partial or complete excisions of the transfected gene in every case. These studies show that quantities of cadmium and zinc that induce metallothioneins also suppress the incidence of deletions in murine cells.

Original languageEnglish (US)
Pages (from-to)9380-9384
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number23
DOIs
StatePublished - 1989

Fingerprint

Cadmium
Zinc
Hypoxanthine Phosphoribosyltransferase
DNA
Genes
Metals
Thymidine Kinase
Metallothionein
Mutagenesis
Clone Cells
Cell Line
Incidence

Keywords

  • heavy metals
  • mutagenesis
  • recombination
  • transfection

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Deletion of stably integrated DNA is suppressed by cadmium and zinc. / Abrams, J. M.; Schimke, E. T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 23, 1989, p. 9380-9384.

Research output: Contribution to journalArticle

@article{be7870925ca24cdc9b93e27a6f06fd5f,
title = "Deletion of stably integrated DNA is suppressed by cadmium and zinc",
abstract = "The bacterial xanthine-guanine phosphoribosyltransferase (GPT) gene was fused to a metal-responsive promoter and transfected into a murine cell line. Clonal transformants harboring metal-responsive or nonresponsive GPT genes (using a thymidine kinase promoter) were then studied for the loss of transfected gene function either during periods of constitutive expression or during periods of induced activity. Nontoxic levels of cadmium and zinc markedly reduced the frequency of mutagenesis in all transfected lines irrespective of transcriptional status. A survey of 17 GPT- clones derived from two original transfectants showed partial or complete excisions of the transfected gene in every case. These studies show that quantities of cadmium and zinc that induce metallothioneins also suppress the incidence of deletions in murine cells.",
keywords = "heavy metals, mutagenesis, recombination, transfection",
author = "Abrams, {J. M.} and Schimke, {E. T.}",
year = "1989",
doi = "10.1073/pnas.86.23.9380",
language = "English (US)",
volume = "86",
pages = "9380--9384",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

TY - JOUR

T1 - Deletion of stably integrated DNA is suppressed by cadmium and zinc

AU - Abrams, J. M.

AU - Schimke, E. T.

PY - 1989

Y1 - 1989

N2 - The bacterial xanthine-guanine phosphoribosyltransferase (GPT) gene was fused to a metal-responsive promoter and transfected into a murine cell line. Clonal transformants harboring metal-responsive or nonresponsive GPT genes (using a thymidine kinase promoter) were then studied for the loss of transfected gene function either during periods of constitutive expression or during periods of induced activity. Nontoxic levels of cadmium and zinc markedly reduced the frequency of mutagenesis in all transfected lines irrespective of transcriptional status. A survey of 17 GPT- clones derived from two original transfectants showed partial or complete excisions of the transfected gene in every case. These studies show that quantities of cadmium and zinc that induce metallothioneins also suppress the incidence of deletions in murine cells.

AB - The bacterial xanthine-guanine phosphoribosyltransferase (GPT) gene was fused to a metal-responsive promoter and transfected into a murine cell line. Clonal transformants harboring metal-responsive or nonresponsive GPT genes (using a thymidine kinase promoter) were then studied for the loss of transfected gene function either during periods of constitutive expression or during periods of induced activity. Nontoxic levels of cadmium and zinc markedly reduced the frequency of mutagenesis in all transfected lines irrespective of transcriptional status. A survey of 17 GPT- clones derived from two original transfectants showed partial or complete excisions of the transfected gene in every case. These studies show that quantities of cadmium and zinc that induce metallothioneins also suppress the incidence of deletions in murine cells.

KW - heavy metals

KW - mutagenesis

KW - recombination

KW - transfection

UR - http://www.scopus.com/inward/record.url?scp=0024306554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024306554&partnerID=8YFLogxK

U2 - 10.1073/pnas.86.23.9380

DO - 10.1073/pnas.86.23.9380

M3 - Article

VL - 86

SP - 9380

EP - 9384

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -