Deletion of the endothelin-A receptor gene within the developing mandible

Louis Bruno Ruest, Rafal Kedzierski, Masashi Yanagisawa, David E. Clouthier

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Signaling from the endothelin-A (Ednra) receptor is responsible for initiating multiple signaling pathways within neural crest cells (NCCs). Loss of this initiation is presumably the basis for the craniofacial defects observed in Ednra -/- embryos. However, it is not known whether continued Ednra signaling in NCC derivatives is required for subsequent development of the lower jaw. To address this question, mice containing loxP recombination sequences flanking a portion of the Ednra gene were bred with transgenic mice that express Cre recombinase under control of a Dlx5/6 enhancer element. We find that while Ednra gene inactivation within the mandibular arch of these Ednra conditional knockout embryos is detectable by embryonic day (E) 10.5, mandibular arch-specific gene expression is normal, as is overall mandible development. These results suggest that while Ednra receptor signaling is crucial for early NCC patterning, subsequent Ednra signaling is not essential for mandible bone development.

Original languageEnglish (US)
Pages (from-to)447-453
Number of pages7
JournalCell and Tissue Research
Volume319
Issue number3
DOIs
StatePublished - Mar 1 2005

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Keywords

  • Craniofacial
  • Cre recombinase
  • Knockout mice
  • Neural crest
  • Transgenic mice
  • loxP

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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