Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona

Christian Dittrich, Christoph J. Burckhardt, Gaudenz Danuser

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs: they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo invitro by a particulate delivery system entirely composed of amino acids.

Original languageEnglish (US)
Pages (from-to)2746-2753
Number of pages8
JournalBiomaterials
Volume33
Issue number9
DOIs
StatePublished - Mar 1 2012

Keywords

  • Corona
  • Drug delivery
  • Nanoparticle
  • Peptide
  • Targeting

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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