Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona

Christian Dittrich, Christoph J. Burckhardt, Gaudenz Danuser

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs: they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo invitro by a particulate delivery system entirely composed of amino acids.

Original languageEnglish (US)
Pages (from-to)2746-2753
Number of pages8
JournalBiomaterials
Volume33
Issue number9
DOIs
StatePublished - Mar 2012

Fingerprint

CHO Cells
Nanoparticles
Peptides
Proteins
Membranes
Transferrin Receptors
Pharmacokinetics
Transferrin
Cell Communication
Phase separation
Pharmaceutical Preparations
Solubility
Drug products
Amino acids
Stabilization
Association reactions
Amino Acids
Costs and Cost Analysis
Degradation
Protein Corona

Keywords

  • Corona
  • Drug delivery
  • Nanoparticle
  • Peptide
  • Targeting

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona. / Dittrich, Christian; Burckhardt, Christoph J.; Danuser, Gaudenz.

In: Biomaterials, Vol. 33, No. 9, 03.2012, p. 2746-2753.

Research output: Contribution to journalArticle

Dittrich, Christian ; Burckhardt, Christoph J. ; Danuser, Gaudenz. / Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona. In: Biomaterials. 2012 ; Vol. 33, No. 9. pp. 2746-2753.
@article{6266df6c584e4d5e9afe7cf73b20dc94,
title = "Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona",
abstract = "Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs: they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo invitro by a particulate delivery system entirely composed of amino acids.",
keywords = "Corona, Drug delivery, Nanoparticle, Peptide, Targeting",
author = "Christian Dittrich and Burckhardt, {Christoph J.} and Gaudenz Danuser",
year = "2012",
month = "3",
doi = "10.1016/j.biomaterials.2011.12.016",
language = "English (US)",
volume = "33",
pages = "2746--2753",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "9",

}

TY - JOUR

T1 - Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona

AU - Dittrich, Christian

AU - Burckhardt, Christoph J.

AU - Danuser, Gaudenz

PY - 2012/3

Y1 - 2012/3

N2 - Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs: they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo invitro by a particulate delivery system entirely composed of amino acids.

AB - Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs: they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo invitro by a particulate delivery system entirely composed of amino acids.

KW - Corona

KW - Drug delivery

KW - Nanoparticle

KW - Peptide

KW - Targeting

UR - http://www.scopus.com/inward/record.url?scp=84855932886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855932886&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.12.016

DO - 10.1016/j.biomaterials.2011.12.016

M3 - Article

C2 - 22226586

AN - SCOPUS:84855932886

VL - 33

SP - 2746

EP - 2753

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 9

ER -