Demonstration of reverse fatty acid transport from rat cardiomyocytes

Byung Hyun Park, Young H Lee, Marlei Walton, Laurence Duplomb, Roger H Unger

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fatty acids flow from adipocytes to nonadipose tissues during fasting and exercise and normally are fully oxidized. To determine if nonadipose tissues can export unoxidized FA when FA influx exceeds oxidation, neonatal cardiomyocytes were cultured in 1 μCi 14C-palmitate in the presence of etomoxir to block oxidation. The cells took up and stored 25% of the radioactivity as 14C-triacylglycerol in 12 h, but 4.5% of the label was released in 3 h and comigrated with 14C-palmitate. Both uptake and release of radioactivity were increased by insulin and reduced by the nonspecific inhibitors of FA transporters phloretin and 4,4′-diisothiocyanatostilbene- 2,2′-disulfonic acid (DIDS). Perfused hearts from etomoxir-treated lean rats released 221 ± 59 nmol/10 min of FA. Hearts from high-fat-fed lean rats released 366 ± 172 nmol/10 min (P < 0.05). Hearts from obese rats released 744 ± 260 and 1,578 ± 630 nmol/10 min at 8 and 12 weeks of age, respectively. Perfusion with insulin increased FA release by 32%. In vitro and ex vivo findings suggest that nonadipose tissues such as myocardium can export FA when the unoxidized lipid content is excessive.

Original languageEnglish (US)
Pages (from-to)1992-1999
Number of pages8
JournalJournal of Lipid Research
Volume45
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Cardiac Myocytes
Rats
Demonstrations
Fatty Acids
Palmitates
Radioactivity
Tissue
Phloretin
Insulin
Oxidation
Adipocytes
Labels
Fasting
Myocardium
Triglycerides
Perfusion
Fats
Lipids
Acids
etomoxir

Keywords

  • Free fatty acid
  • Insulin
  • Liporegulation
  • Triacylglycerol

ASJC Scopus subject areas

  • Endocrinology

Cite this

Demonstration of reverse fatty acid transport from rat cardiomyocytes. / Park, Byung Hyun; Lee, Young H; Walton, Marlei; Duplomb, Laurence; Unger, Roger H.

In: Journal of Lipid Research, Vol. 45, No. 11, 11.2004, p. 1992-1999.

Research output: Contribution to journalArticle

Park, Byung Hyun ; Lee, Young H ; Walton, Marlei ; Duplomb, Laurence ; Unger, Roger H. / Demonstration of reverse fatty acid transport from rat cardiomyocytes. In: Journal of Lipid Research. 2004 ; Vol. 45, No. 11. pp. 1992-1999.
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