Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells

Senthil Kumar Venugopal, Sridevi Devaraj, Ivan Yuhanna, Philip Shaul, Ishwarlal Jialal

Research output: Contribution to journalArticle

687 Scopus citations

Abstract

Background - C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis. Methods and Results - We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs. Conclusion - CRP causes a direct reduction in eNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.

Original languageEnglish (US)
Pages (from-to)1439-1441
Number of pages3
JournalCirculation
Volume106
Issue number12
DOIs
StatePublished - Sep 17 2002

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Keywords

  • C-reactive protein
  • Endothelium
  • Inflammation
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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