Dendritic cells produce macrophage inflammatory protein-1γ, a new member of the CC chemokine family

Mansour Mohamadzadeh, Alexander N. Poltorak, Paul R. Bergstresser, Bruce Beutler, Akira Takashima

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Langerhans cells (LC) are skin-specific members of the dendritic cell (DC) family. DC are unique among APC for their capacity to activate immunologically naive T cells, but little is known about their chemotactic recruitment of T cells. We now report that LC produce macrophage inflammatory protein-1γ (MIP-1γ), a newly identified CC chemokine. MIP-1γ mRNA was detected in epidermal cells freshly procured from BALB/c mice, and depletion of I-A+ epidermal cells (i.e., LC) abrogated that expression. MIP-1γ mRNA was detected in the XS52 LC-like DC line as well as by 4F7+ splenic DC and granulocyte-macrophage CSF-propagated bone marrow DC. XS52 DC culture supernatants contained 9 and 10.5 kDa immunoreactivities with anti-MIP-1γ Abs. We observed in Boyden chamber assays that 1) XS52 DC supernatant (added to the lower chambers) induced significant migration by splenic T cells; 2) this migration was blocked by the addition of anti-MIP-1γ in the lower chambers or by rMIP-1γ in the upper chambers; and 3) comparable migration occurred in both CD4+ and CD8+ T cells and in both activated and nonactivated T cells. We conclude that mouse DC (including LC) have the capacity to elaborate the novel CC chemokine MIP-1γ, suggesting the active participation of DC in recruiting T cells before activation.

Original languageEnglish (US)
Pages (from-to)3102-3106
Number of pages5
JournalJournal of Immunology
Volume156
Issue number9
StatePublished - May 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Mohamadzadeh, M., Poltorak, A. N., Bergstresser, P. R., Beutler, B., & Takashima, A. (1996). Dendritic cells produce macrophage inflammatory protein-1γ, a new member of the CC chemokine family. Journal of Immunology, 156(9), 3102-3106.