Abstract
The buoyant densities of mouse immunoglobulins were determined by isopycnic centrifugation in phosphate-buffered cesium chloride using β-galactosidase as marker. The buoyant densities of IgG, TEPC 15 IgA, secreted IgM, and MOPC 104E IgM were consistent with their carbohydrate contents both in the presence and the absence of the nonionic detergent, Nonidet P-40. Intracellular IgM from spleen cell lysates had a buoyant density corresponding to a carbohydrate content of 6%. Membrane IgM from detergent lysates of spleen cells was less dense than either intracellular or secreted IgM in the presence of detergent. The IgD-like membrane molecules were more dense than membrane IgM.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-152 |
| Number of pages | 8 |
| Journal | Biochemistry |
| Volume | 16 |
| Issue number | 1 |
| State | Published - 1977 |
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- Biochemistry
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Density differences between membrane and secreted immunoglobulins of murine splenocytes. / Melcher, Ulrich; Uhr, Jonathan W.
In: Biochemistry, Vol. 16, No. 1, 1977, p. 145-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Density differences between membrane and secreted immunoglobulins of murine splenocytes
AU - Melcher, Ulrich
AU - Uhr, Jonathan W.
PY - 1977
Y1 - 1977
N2 - The buoyant densities of mouse immunoglobulins were determined by isopycnic centrifugation in phosphate-buffered cesium chloride using β-galactosidase as marker. The buoyant densities of IgG, TEPC 15 IgA, secreted IgM, and MOPC 104E IgM were consistent with their carbohydrate contents both in the presence and the absence of the nonionic detergent, Nonidet P-40. Intracellular IgM from spleen cell lysates had a buoyant density corresponding to a carbohydrate content of 6%. Membrane IgM from detergent lysates of spleen cells was less dense than either intracellular or secreted IgM in the presence of detergent. The IgD-like membrane molecules were more dense than membrane IgM.
AB - The buoyant densities of mouse immunoglobulins were determined by isopycnic centrifugation in phosphate-buffered cesium chloride using β-galactosidase as marker. The buoyant densities of IgG, TEPC 15 IgA, secreted IgM, and MOPC 104E IgM were consistent with their carbohydrate contents both in the presence and the absence of the nonionic detergent, Nonidet P-40. Intracellular IgM from spleen cell lysates had a buoyant density corresponding to a carbohydrate content of 6%. Membrane IgM from detergent lysates of spleen cells was less dense than either intracellular or secreted IgM in the presence of detergent. The IgD-like membrane molecules were more dense than membrane IgM.
UR - http://www.scopus.com/inward/record.url?scp=0017365630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017365630&partnerID=8YFLogxK
M3 - Article
VL - 16
SP - 145
EP - 152
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 1
ER -