Background: Essential tremor involves the cerebellum, yet quantitative analysis of dentate nucleus neurons has not been conducted. Objectives: To quantitatively compare neuronal density or neuronal number in the dentate nucleus of essential tremor versus age-matched controls. Methods: Using a 7-μm thick Luxol fast blue hematoxylin and eosin-stained paraffin section, dentate nucleus neuronal density (neurons/mm2) was determined in 25 essential tremor cases and 25 controls. We also applied a stereological approach in a subset of four essential tremor cases and four controls to estimate total dentate nucleus neuronal number. Results: Dentate nucleus neuronal density did not differ between essential tremor cases and controls (P = 0.44). Total dentate nucleus neuronal number correlated with neuronal density (P = 0.007) and did not differ between essential tremor cases and controls (P = 0.95). Conclusions: Neuronal loss, observed in the Purkinje cell population in essential tremor, did not seem to similarly involve the dentate nucleus in essential tremor.
- essential tremor; cerebellum; dentate neuron; stereology; pathology
ASJC Scopus subject areas
- Clinical Neurology