Dentin permeability reduction by a sequential application of calcium and fluoride-phosphate solutions

Takashi Komabayashi, Yohji Imai, Chul Ahn, Laurence C. Chow, Shozo Takagi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: A sequential topical application of calcium and fluoride-phosphate solutions was reported to occlude open dentin tubules, mainly with fluoroapatite precipitates by a rapid ionic reaction, and to be effective at treating dentin hypersensitivity. However, its ability to reduce dentin permeability (Lp) is unknown. The aim of this in vitro study was to evaluate the effect of this treatment on Lp. Methods: Nine extracted human third molars were sectioned transversely to obtain 0.5 mm-thick discs, which were then etched and rinsed. Aqueous solutions of 5% (w/w) disodium phosphate containing 0.3% (w/w) sodium fluoride (A) and 10% (w/w) calcium chloride (B) were prepared. The sequential application of the A&B solutions was repeated three times on each disc, which was then rinsed with distilled water. The Lp of the discs was measured before and after the application using a modified Pashley's fluid flow measuring system. The differences in the Lp values between the conditions before and after the solution applications were analysed using a generalized estimating equation method and paired t-test. Scanning Electron Microscopy (SEM) was used to observe the dentin surfaces. Results: All nine discs consistently indicated reduced Lp following the application of the A&B solutions. There was a significant decrease in the mean Lp [μL/(cm2 s cm H 2O)] from baseline (-0.27 ± 0.25, p = 0.011). Overall, an average decrease of 34% Lp occurred after the application of the A&B solutions. SEM observation indicated that the reaction products covered the entire dentin disc surface. Conclusion: The application of the A&B solutions was effective at reducing the Lp of the dentin discs.

Original languageEnglish (US)
Pages (from-to)736-741
Number of pages6
JournalJournal of Dentistry
Volume38
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Dentin Permeability
Dentin
Electron Scanning Microscopy
Dentin Sensitivity
Sodium Fluoride
Calcium Chloride
Third Molar
calcium phosphate
Phosphates
Observation
Water

Keywords

  • Calcium chloride
  • Dentin hypersensitivity
  • Dentin permeability
  • Dentin tubules
  • Desensitizing agents
  • Disodium phosphate
  • Sequential application
  • Sodium fluoride

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Dentin permeability reduction by a sequential application of calcium and fluoride-phosphate solutions. / Komabayashi, Takashi; Imai, Yohji; Ahn, Chul; Chow, Laurence C.; Takagi, Shozo.

In: Journal of Dentistry, Vol. 38, No. 9, 09.2010, p. 736-741.

Research output: Contribution to journalArticle

Komabayashi, Takashi ; Imai, Yohji ; Ahn, Chul ; Chow, Laurence C. ; Takagi, Shozo. / Dentin permeability reduction by a sequential application of calcium and fluoride-phosphate solutions. In: Journal of Dentistry. 2010 ; Vol. 38, No. 9. pp. 736-741.
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abstract = "Objective: A sequential topical application of calcium and fluoride-phosphate solutions was reported to occlude open dentin tubules, mainly with fluoroapatite precipitates by a rapid ionic reaction, and to be effective at treating dentin hypersensitivity. However, its ability to reduce dentin permeability (Lp) is unknown. The aim of this in vitro study was to evaluate the effect of this treatment on Lp. Methods: Nine extracted human third molars were sectioned transversely to obtain 0.5 mm-thick discs, which were then etched and rinsed. Aqueous solutions of 5{\%} (w/w) disodium phosphate containing 0.3{\%} (w/w) sodium fluoride (A) and 10{\%} (w/w) calcium chloride (B) were prepared. The sequential application of the A&B solutions was repeated three times on each disc, which was then rinsed with distilled water. The Lp of the discs was measured before and after the application using a modified Pashley's fluid flow measuring system. The differences in the Lp values between the conditions before and after the solution applications were analysed using a generalized estimating equation method and paired t-test. Scanning Electron Microscopy (SEM) was used to observe the dentin surfaces. Results: All nine discs consistently indicated reduced Lp following the application of the A&B solutions. There was a significant decrease in the mean Lp [μL/(cm2 s cm H 2O)] from baseline (-0.27 ± 0.25, p = 0.011). Overall, an average decrease of 34{\%} Lp occurred after the application of the A&B solutions. SEM observation indicated that the reaction products covered the entire dentin disc surface. Conclusion: The application of the A&B solutions was effective at reducing the Lp of the dentin discs.",
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AU - Takagi, Shozo

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AB - Objective: A sequential topical application of calcium and fluoride-phosphate solutions was reported to occlude open dentin tubules, mainly with fluoroapatite precipitates by a rapid ionic reaction, and to be effective at treating dentin hypersensitivity. However, its ability to reduce dentin permeability (Lp) is unknown. The aim of this in vitro study was to evaluate the effect of this treatment on Lp. Methods: Nine extracted human third molars were sectioned transversely to obtain 0.5 mm-thick discs, which were then etched and rinsed. Aqueous solutions of 5% (w/w) disodium phosphate containing 0.3% (w/w) sodium fluoride (A) and 10% (w/w) calcium chloride (B) were prepared. The sequential application of the A&B solutions was repeated three times on each disc, which was then rinsed with distilled water. The Lp of the discs was measured before and after the application using a modified Pashley's fluid flow measuring system. The differences in the Lp values between the conditions before and after the solution applications were analysed using a generalized estimating equation method and paired t-test. Scanning Electron Microscopy (SEM) was used to observe the dentin surfaces. Results: All nine discs consistently indicated reduced Lp following the application of the A&B solutions. There was a significant decrease in the mean Lp [μL/(cm2 s cm H 2O)] from baseline (-0.27 ± 0.25, p = 0.011). Overall, an average decrease of 34% Lp occurred after the application of the A&B solutions. SEM observation indicated that the reaction products covered the entire dentin disc surface. Conclusion: The application of the A&B solutions was effective at reducing the Lp of the dentin discs.

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KW - Sequential application

KW - Sodium fluoride

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