Dependence of gonadotropin-induced steroidogenesis upon RNA and protein synthesis in the interstitial cells of the rat testis

Carole Mendelson, Maria Dufau, Kevin Catt

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The effects of inhibitors of RNA and protein synthesis upon gonadotropic stimulation of testosterone and cyclic AMP production by the Leydig cell were investigated in vitro with enzyme-dispersed interstitial cells of the rat testis. The testosterone response to human chorionic gonadotropin was abolished by cycloheximide and puromycin, and was markedly reduced by actinomycin D and cordycepin. During 3-h times studies, cycloheximide caused complete inhibition of subsequent steroid production when added at times up to 90 min after the commencement of incubation with human chorionic gonadotropin. Actinomycin D did not completely abolish the testosterone response when added at zero time, and became progressively less effective when added at later times during the incubation period. The stimulation of steroidogenesis by dibutyryl cyclic AMP was also completely abolished by cycloheximide and puromycin, and was siginificantly reduced by actinomycin D and cordycepin. By contrast with the marked inhibition od steroid production by cycloheximide and actinomycin D, the formation of cyclic AMP during human chorionic gonadotropin stimulation was relatively unaffected by either inhibitor. These results indicate that the stimulation of testosterone production by the Leyding cell in response to gonadotropins and dibutyryl cyclic AMP is dependent upon the synthesis of new RNA and protein molecules. Thus, effects of gonadotropin and cyclic AMP upon both transcriptional and translational processes appear to be essential intermediate steps in the activation of testicular steroidogenesis. The rapid and complete abolition of subsequent steroid synthesis following addition of cycloheximide or puromycin to human chorionic gonadotropin-stimulated interstitial cells suggests that e relatively labile protein is formed during gonadotropin actions.

Original languageEnglish (US)
Pages (from-to)222-230
Number of pages9
JournalBBA - General Subjects
Volume411
Issue number2
DOIs
StatePublished - Dec 5 1975

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Cycloheximide
Gonadotropins
Testis
Rats
Dactinomycin
Chorionic Gonadotropin
Puromycin
Cells
RNA
Testosterone
Cyclic AMP
Bucladesine
Steroids
Proteins
Nucleic Acid Synthesis Inhibitors
Time and motion study
Protein Synthesis Inhibitors
Time and Motion Studies
Leydig Cells
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Dependence of gonadotropin-induced steroidogenesis upon RNA and protein synthesis in the interstitial cells of the rat testis. / Mendelson, Carole; Dufau, Maria; Catt, Kevin.

In: BBA - General Subjects, Vol. 411, No. 2, 05.12.1975, p. 222-230.

Research output: Contribution to journalArticle

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