Depolarization-independent net uptake of calcium into clonal insulin-releasing cells exposed to glucose

Erik Gylfe, Tommy Andersson, Patrik Rorsman, Håkan Abrahamsson, Per Arkhammar, Per Hellman, Bo Hellman, Herbert K. Oie, Adi F. Gazdar

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Abstract

Insulin release, net fluxes of Ca2+, and glucose metabolism were studied in a clonal cell line (RINmSF) established from a transplantable rat islet tumor. The insulin content amounted to only 0.03% of that of the total protein and decreased even further with subsequent passages. The insulin secretion was as high as 10 to 20% of the total hormone content per hour. Insulin release was stimulated by K+ depolarization but not by exposure to glucose. In contrast to this secretory pattern, glucose but not K+ stimulated the net uptake of Ca2+ at micromolar concentrations of the ion. The glucose effect was not mimicked by 20 mM 3-O-methylglucose. It was as pronounced at 1 mM as at 20 mM of the sugar and corresponded to an uptake of 119 fmol cm-2 s-1. Glucose metabolism was typical for tumor cells with a high glycolytic flux and an oxidationtoutilization ratio as low as 0.05-0.15. Maximal oxidative degradation was attained already at l mM. This concentration was also equivalent to the Km for glucose utilization, indicating a substantial left-hand shift of the normal dose-response curve. It is suggested that glucose induces a depolarizationindependent net uptake of Ca2+ by favouring intracellular buffering of the cation.

Original languageEnglish (US)
Pages (from-to)927-937
Number of pages11
JournalBioscience Reports
Volume3
Issue number10
DOIs
Publication statusPublished - Oct 1983

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ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gylfe, E., Andersson, T., Rorsman, P., Abrahamsson, H., Arkhammar, P., Hellman, P., ... Gazdar, A. F. (1983). Depolarization-independent net uptake of calcium into clonal insulin-releasing cells exposed to glucose. Bioscience Reports, 3(10), 927-937. https://doi.org/10.1007/BF01140662