Deposition of fibronectin on material surfaces exposed to plasma: Quantitative and biological studies

F. Grinnell, T. V. Phan

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Experiments were carried out to characterize plasma fibronectin deposition onto material surfaces exposed to plasma solutions. Under nonclotting conditions, the amount of fibronectin adsorption of the surfaces, determined by an indirect radioactive antibody assay, was maximal at low plasma concentrations (0.1%). At higher concentrations of plasma, other plasma proteins appeared to compete with and inhibit adsorption of fibronectin. Biological activity (fibronectin-promoted cell spreading) was also greatest at low plasma concentrations and decreased as the plasma concentration was raised. When surfaces were exposed to plasma under clotting conditions (i.e., addition of Ca2+ and thrombin) fibronectin deposition on the surfaces and biological activity remained constant or increased as the plasma concentration was raised. Based on indirect immunofluorescent antibody assays, the fibronectin deposited from clotting plasma appeared to be in a punctate distribution over the entire material surface and occasionally was associated with discrete fibrillar structures. The increased deposition of fibronectin from clotting plasma compared to nonclotting plasma (approximately a 10-fold difference with 10% plasma) was partially a result of covalent crosslinking of fibronectin to fibrin based upon studies with putrescine added to inhibit crosslinking during clotting. On the other hand, the increase in biologial activity that occurred if the surfaces were exposed to clotting plasma was completely inhibited by putrescine, indicating that fibronectin has to be crosslinked to fibrin to have biological activity under these conditions. Finally, fibronectin deposition also occurred on surfaces exposed to whole blood and was markedly enhanced when clotting occurred.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalJournal of Cellular Physiology
Volume116
Issue number3
StatePublished - 1983

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Fibronectins
Plasmas
Bioactivity
Putrescine
Fibrin
Crosslinking
Assays
Adsorption
Plasma deposition
Antibodies
Thrombin
Blood Proteins
Blood

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Deposition of fibronectin on material surfaces exposed to plasma : Quantitative and biological studies. / Grinnell, F.; Phan, T. V.

In: Journal of Cellular Physiology, Vol. 116, No. 3, 1983, p. 289-296.

Research output: Contribution to journalArticle

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