Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x fisher 344 cross

L. C. Solberg, N. Ahmadiyeh, A. E. Baum, M. H. Vitaterna, J. S. Takahashi, F. W. Turek, E. E. Redei

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Depression is a heritable disorder that is often precipitated by stress. Abnormalities of the stress-reactive hypothalamic-pituitary-adrenal (HPA) axis are also common in depressed patients. In animal models, the forced swim test (FST) is the most frequently used test of depressive-like behavior. We have used a proposed animal model of depression, the Wistar Kyoto (WKY) rat, to investigate the relationship as well as the mode of inheritance of FST behaviors and HPA measures. Through reciprocal breeding of WKY and F344 parent strains and brother-sister breeding of the F1 generation, we obtained 486 F2 animals. Parent, F1 and F2 animals were tested in the FST. Blood samples were collected for determination of basal and stress (10-min restraint) plasma corticosterone (CORT) levels, and adrenal weights were measured. We found that all measures were heritable to some extent and that this heritability was highly sex dependent. Both correlation and factor analyses of the F2 generation data demonstrate that FST behavior and HPA axis measures are not directly related. Thus, the underlying genetic components of depressive-like behavior and HPA axis abnormalities are likely to be disparate in the segregating F2 generation of a WKYxF344 cross.

Original languageEnglish (US)
Pages (from-to)423-433
Number of pages11
JournalMolecular Psychiatry
Volume8
Issue number4
DOIs
StatePublished - 2003

Fingerprint

Breeding
Siblings
Animal Models
Depression
Inbred WKY Rats
Corticosterone
Statistical Factor Analysis
Weights and Measures

Keywords

  • Adrenal weight
  • Corticosterone
  • Forced swim behavior
  • Genetics
  • Heritability
  • Hypothalamic-pituitary-adrenal axis
  • Strain differences
  • Stress response

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health

Cite this

Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x fisher 344 cross. / Solberg, L. C.; Ahmadiyeh, N.; Baum, A. E.; Vitaterna, M. H.; Takahashi, J. S.; Turek, F. W.; Redei, E. E.

In: Molecular Psychiatry, Vol. 8, No. 4, 2003, p. 423-433.

Research output: Contribution to journalArticle

Solberg, L. C. ; Ahmadiyeh, N. ; Baum, A. E. ; Vitaterna, M. H. ; Takahashi, J. S. ; Turek, F. W. ; Redei, E. E. / Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x fisher 344 cross. In: Molecular Psychiatry. 2003 ; Vol. 8, No. 4. pp. 423-433.
@article{0b4eddb9fed2482cab714f4f4f0bc34c,
title = "Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x fisher 344 cross",
abstract = "Depression is a heritable disorder that is often precipitated by stress. Abnormalities of the stress-reactive hypothalamic-pituitary-adrenal (HPA) axis are also common in depressed patients. In animal models, the forced swim test (FST) is the most frequently used test of depressive-like behavior. We have used a proposed animal model of depression, the Wistar Kyoto (WKY) rat, to investigate the relationship as well as the mode of inheritance of FST behaviors and HPA measures. Through reciprocal breeding of WKY and F344 parent strains and brother-sister breeding of the F1 generation, we obtained 486 F2 animals. Parent, F1 and F2 animals were tested in the FST. Blood samples were collected for determination of basal and stress (10-min restraint) plasma corticosterone (CORT) levels, and adrenal weights were measured. We found that all measures were heritable to some extent and that this heritability was highly sex dependent. Both correlation and factor analyses of the F2 generation data demonstrate that FST behavior and HPA axis measures are not directly related. Thus, the underlying genetic components of depressive-like behavior and HPA axis abnormalities are likely to be disparate in the segregating F2 generation of a WKYxF344 cross.",
keywords = "Adrenal weight, Corticosterone, Forced swim behavior, Genetics, Heritability, Hypothalamic-pituitary-adrenal axis, Strain differences, Stress response",
author = "Solberg, {L. C.} and N. Ahmadiyeh and Baum, {A. E.} and Vitaterna, {M. H.} and Takahashi, {J. S.} and Turek, {F. W.} and Redei, {E. E.}",
year = "2003",
doi = "10.1038/sj.mp.4001255",
language = "English (US)",
volume = "8",
pages = "423--433",
journal = "Molecular Psychiatry",
issn = "1359-4184",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Depressive-like behavior and stress reactivity are independent traits in a Wistar Kyoto x fisher 344 cross

AU - Solberg, L. C.

AU - Ahmadiyeh, N.

AU - Baum, A. E.

AU - Vitaterna, M. H.

AU - Takahashi, J. S.

AU - Turek, F. W.

AU - Redei, E. E.

PY - 2003

Y1 - 2003

N2 - Depression is a heritable disorder that is often precipitated by stress. Abnormalities of the stress-reactive hypothalamic-pituitary-adrenal (HPA) axis are also common in depressed patients. In animal models, the forced swim test (FST) is the most frequently used test of depressive-like behavior. We have used a proposed animal model of depression, the Wistar Kyoto (WKY) rat, to investigate the relationship as well as the mode of inheritance of FST behaviors and HPA measures. Through reciprocal breeding of WKY and F344 parent strains and brother-sister breeding of the F1 generation, we obtained 486 F2 animals. Parent, F1 and F2 animals were tested in the FST. Blood samples were collected for determination of basal and stress (10-min restraint) plasma corticosterone (CORT) levels, and adrenal weights were measured. We found that all measures were heritable to some extent and that this heritability was highly sex dependent. Both correlation and factor analyses of the F2 generation data demonstrate that FST behavior and HPA axis measures are not directly related. Thus, the underlying genetic components of depressive-like behavior and HPA axis abnormalities are likely to be disparate in the segregating F2 generation of a WKYxF344 cross.

AB - Depression is a heritable disorder that is often precipitated by stress. Abnormalities of the stress-reactive hypothalamic-pituitary-adrenal (HPA) axis are also common in depressed patients. In animal models, the forced swim test (FST) is the most frequently used test of depressive-like behavior. We have used a proposed animal model of depression, the Wistar Kyoto (WKY) rat, to investigate the relationship as well as the mode of inheritance of FST behaviors and HPA measures. Through reciprocal breeding of WKY and F344 parent strains and brother-sister breeding of the F1 generation, we obtained 486 F2 animals. Parent, F1 and F2 animals were tested in the FST. Blood samples were collected for determination of basal and stress (10-min restraint) plasma corticosterone (CORT) levels, and adrenal weights were measured. We found that all measures were heritable to some extent and that this heritability was highly sex dependent. Both correlation and factor analyses of the F2 generation data demonstrate that FST behavior and HPA axis measures are not directly related. Thus, the underlying genetic components of depressive-like behavior and HPA axis abnormalities are likely to be disparate in the segregating F2 generation of a WKYxF344 cross.

KW - Adrenal weight

KW - Corticosterone

KW - Forced swim behavior

KW - Genetics

KW - Heritability

KW - Hypothalamic-pituitary-adrenal axis

KW - Strain differences

KW - Stress response

UR - http://www.scopus.com/inward/record.url?scp=0037615175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037615175&partnerID=8YFLogxK

U2 - 10.1038/sj.mp.4001255

DO - 10.1038/sj.mp.4001255

M3 - Article

C2 - 12740600

AN - SCOPUS:0037615175

VL - 8

SP - 423

EP - 433

JO - Molecular Psychiatry

JF - Molecular Psychiatry

SN - 1359-4184

IS - 4

ER -