Derivation and Differentiation of Human Embryonic Germ Cells

Michael J. Shamblott, Candace L. Kerr, Joyce Axelman, John W. Littlefield, Gregory O. Clark, Ethan S. Patterson, Russell C. Addis, Jennifer N. Kraszewski, Kathleen C. Kent, John D. Gearhart

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. PGCs are the sole means of genetic transmission between parent and offspring, as they generate eggs and sperm. Rather than having a previously determined fate, cells in this location receive external signals to further differentiate into PGCs, as demonstrated by the observation that transplantation of cells from other parts of the epiblast to this region can take on a PGC fate. PGCs are associated with dorsal mesenteries, and are translocated to genital ridges. Both cellular migration and association with moving tissues cause the migration of PGCs. In absence of inhibitory signals, female PGCs undergo cogenesis. Although not as thoroughly studied, much is known regarding the migratory path of human PGCs, including their association with gut endoderm and migration into developing genital ridges. PGCs do not survive well under standard tissue culture conditions and are not pluripotent stem cells in vivo or in vitro. Cells that retain a high capacity for cell proliferation and express markers of multiple lineages can be isolated from embryoid bodies (EBs), and can be used in a variety of in vitro and in vivo differentiation paradigms.

Original languageEnglish (US)
Title of host publicationHandbook of Stem Cells
PublisherElsevier Inc.
Pages459-470
Number of pages12
Volume1
ISBN (Print)9780080533735, 9780124366435
DOIs
StatePublished - Sep 14 2004

Fingerprint

Germ Cells
Cells
Tissue culture
Cell proliferation
Stem cells
Tissue
Embryoid Bodies
Embryonic Germ Cells
Germ Layers
Pluripotent Stem Cells
Endoderm
Mesentery
Cell Transplantation
Eggs
Spermatozoa
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Shamblott, M. J., Kerr, C. L., Axelman, J., Littlefield, J. W., Clark, G. O., Patterson, E. S., ... Gearhart, J. D. (2004). Derivation and Differentiation of Human Embryonic Germ Cells. In Handbook of Stem Cells (Vol. 1, pp. 459-470). Elsevier Inc.. https://doi.org/10.1016/B978-012436643-5/50052-3

Derivation and Differentiation of Human Embryonic Germ Cells. / Shamblott, Michael J.; Kerr, Candace L.; Axelman, Joyce; Littlefield, John W.; Clark, Gregory O.; Patterson, Ethan S.; Addis, Russell C.; Kraszewski, Jennifer N.; Kent, Kathleen C.; Gearhart, John D.

Handbook of Stem Cells. Vol. 1 Elsevier Inc., 2004. p. 459-470.

Research output: Chapter in Book/Report/Conference proceedingChapter

Shamblott, MJ, Kerr, CL, Axelman, J, Littlefield, JW, Clark, GO, Patterson, ES, Addis, RC, Kraszewski, JN, Kent, KC & Gearhart, JD 2004, Derivation and Differentiation of Human Embryonic Germ Cells. in Handbook of Stem Cells. vol. 1, Elsevier Inc., pp. 459-470. https://doi.org/10.1016/B978-012436643-5/50052-3
Shamblott MJ, Kerr CL, Axelman J, Littlefield JW, Clark GO, Patterson ES et al. Derivation and Differentiation of Human Embryonic Germ Cells. In Handbook of Stem Cells. Vol. 1. Elsevier Inc. 2004. p. 459-470 https://doi.org/10.1016/B978-012436643-5/50052-3
Shamblott, Michael J. ; Kerr, Candace L. ; Axelman, Joyce ; Littlefield, John W. ; Clark, Gregory O. ; Patterson, Ethan S. ; Addis, Russell C. ; Kraszewski, Jennifer N. ; Kent, Kathleen C. ; Gearhart, John D. / Derivation and Differentiation of Human Embryonic Germ Cells. Handbook of Stem Cells. Vol. 1 Elsevier Inc., 2004. pp. 459-470
@inbook{f4282fd5222a40ebab3f22bf491b84bd,
title = "Derivation and Differentiation of Human Embryonic Germ Cells",
abstract = "Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. PGCs are the sole means of genetic transmission between parent and offspring, as they generate eggs and sperm. Rather than having a previously determined fate, cells in this location receive external signals to further differentiate into PGCs, as demonstrated by the observation that transplantation of cells from other parts of the epiblast to this region can take on a PGC fate. PGCs are associated with dorsal mesenteries, and are translocated to genital ridges. Both cellular migration and association with moving tissues cause the migration of PGCs. In absence of inhibitory signals, female PGCs undergo cogenesis. Although not as thoroughly studied, much is known regarding the migratory path of human PGCs, including their association with gut endoderm and migration into developing genital ridges. PGCs do not survive well under standard tissue culture conditions and are not pluripotent stem cells in vivo or in vitro. Cells that retain a high capacity for cell proliferation and express markers of multiple lineages can be isolated from embryoid bodies (EBs), and can be used in a variety of in vitro and in vivo differentiation paradigms.",
author = "Shamblott, {Michael J.} and Kerr, {Candace L.} and Joyce Axelman and Littlefield, {John W.} and Clark, {Gregory O.} and Patterson, {Ethan S.} and Addis, {Russell C.} and Kraszewski, {Jennifer N.} and Kent, {Kathleen C.} and Gearhart, {John D.}",
year = "2004",
month = "9",
day = "14",
doi = "10.1016/B978-012436643-5/50052-3",
language = "English (US)",
isbn = "9780080533735",
volume = "1",
pages = "459--470",
booktitle = "Handbook of Stem Cells",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Derivation and Differentiation of Human Embryonic Germ Cells

AU - Shamblott, Michael J.

AU - Kerr, Candace L.

AU - Axelman, Joyce

AU - Littlefield, John W.

AU - Clark, Gregory O.

AU - Patterson, Ethan S.

AU - Addis, Russell C.

AU - Kraszewski, Jennifer N.

AU - Kent, Kathleen C.

AU - Gearhart, John D.

PY - 2004/9/14

Y1 - 2004/9/14

N2 - Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. PGCs are the sole means of genetic transmission between parent and offspring, as they generate eggs and sperm. Rather than having a previously determined fate, cells in this location receive external signals to further differentiate into PGCs, as demonstrated by the observation that transplantation of cells from other parts of the epiblast to this region can take on a PGC fate. PGCs are associated with dorsal mesenteries, and are translocated to genital ridges. Both cellular migration and association with moving tissues cause the migration of PGCs. In absence of inhibitory signals, female PGCs undergo cogenesis. Although not as thoroughly studied, much is known regarding the migratory path of human PGCs, including their association with gut endoderm and migration into developing genital ridges. PGCs do not survive well under standard tissue culture conditions and are not pluripotent stem cells in vivo or in vitro. Cells that retain a high capacity for cell proliferation and express markers of multiple lineages can be isolated from embryoid bodies (EBs), and can be used in a variety of in vitro and in vivo differentiation paradigms.

AB - Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. PGCs are the sole means of genetic transmission between parent and offspring, as they generate eggs and sperm. Rather than having a previously determined fate, cells in this location receive external signals to further differentiate into PGCs, as demonstrated by the observation that transplantation of cells from other parts of the epiblast to this region can take on a PGC fate. PGCs are associated with dorsal mesenteries, and are translocated to genital ridges. Both cellular migration and association with moving tissues cause the migration of PGCs. In absence of inhibitory signals, female PGCs undergo cogenesis. Although not as thoroughly studied, much is known regarding the migratory path of human PGCs, including their association with gut endoderm and migration into developing genital ridges. PGCs do not survive well under standard tissue culture conditions and are not pluripotent stem cells in vivo or in vitro. Cells that retain a high capacity for cell proliferation and express markers of multiple lineages can be isolated from embryoid bodies (EBs), and can be used in a variety of in vitro and in vivo differentiation paradigms.

UR - http://www.scopus.com/inward/record.url?scp=84944414519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944414519&partnerID=8YFLogxK

U2 - 10.1016/B978-012436643-5/50052-3

DO - 10.1016/B978-012436643-5/50052-3

M3 - Chapter

AN - SCOPUS:84944414519

SN - 9780080533735

SN - 9780124366435

VL - 1

SP - 459

EP - 470

BT - Handbook of Stem Cells

PB - Elsevier Inc.

ER -