TY - JOUR
T1 - Derivation of Intermediate Pluripotent Stem Cells Amenable to Primordial Germ Cell Specification
AU - Yu, Leqian
AU - Wei, Yulei
AU - Sun, Hai Xi
AU - Mahdi, Ahmed K.
AU - Pinzon Arteaga, Carlos A.
AU - Sakurai, Masahiro
AU - Schmitz, Daniel A.
AU - Zheng, Canbin
AU - Ballard, Emily D.
AU - Li, Jie
AU - Tanaka, Noriko
AU - Kohara, Aoi
AU - Okamura, Daiji
AU - Mutto, Adrian A.
AU - Gu, Ying
AU - Ross, Pablo J.
AU - Wu, Jun
N1 - Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2021/3/4
Y1 - 2021/3/4
N2 - Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and are capable of contributing to intra- or inter-species chimeras in vivo. XPSCs represent a pluripotency state between naive and primed pluripotency and harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency. XPSCs open new avenues for studying mammalian pluripotency and dissecting the molecular mechanisms governing PGC specification. Our method may be broadly applicable for the derivation of analogous stem cells from other mammalian species. Yu, Wu, and colleagues report the derivation of intermediate PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and capable of contributing to intra- or inter-species chimeras in vivo. XPSCs harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency.
AB - Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and are capable of contributing to intra- or inter-species chimeras in vivo. XPSCs represent a pluripotency state between naive and primed pluripotency and harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency. XPSCs open new avenues for studying mammalian pluripotency and dissecting the molecular mechanisms governing PGC specification. Our method may be broadly applicable for the derivation of analogous stem cells from other mammalian species. Yu, Wu, and colleagues report the derivation of intermediate PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for direct PGC-like cell induction in vitro and capable of contributing to intra- or inter-species chimeras in vivo. XPSCs harbor molecular, cellular, and phenotypic features characteristic of formative pluripotency.
KW - Pluripotency
KW - chimeras
KW - formative pluripotency
KW - horse embryonic stem cells
KW - induced pluripotent stem cells
KW - intermediate pluripotent stem cells
KW - interspecies chimeras
KW - primoridial germ cells
UR - http://www.scopus.com/inward/record.url?scp=85097905243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85097905243&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2020.11.003
DO - 10.1016/j.stem.2020.11.003
M3 - Article
C2 - 33271070
AN - SCOPUS:85097905243
SN - 1934-5909
VL - 28
SP - 550-567.e12
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 3
ER -