TY - JOUR
T1 - Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency
AU - Yang, Yang
AU - Liu, Bei
AU - Xu, Jun
AU - Wang, Jinlin
AU - Wu, Jun
AU - Shi, Cheng
AU - Xu, Yaxing
AU - Dong, Jiebin
AU - Wang, Chengyan
AU - Lai, Weifeng
AU - Zhu, Jialiang
AU - Xiong, Liang
AU - Zhu, Dicong
AU - Li, Xiang
AU - Yang, Weifeng
AU - Yamauchi, Takayoshi
AU - Sugawara, Atsushi
AU - Li, Zhongwei
AU - Sun, Fangyuan
AU - Li, Xiangyun
AU - Li, Chen
AU - He, Aibin
AU - Du, Yaqin
AU - Wang, Ting
AU - Zhao, Chaoran
AU - Li, Haibo
AU - Chi, Xiaochun
AU - Zhang, Hongquan
AU - Liu, Yifang
AU - Li, Cheng
AU - Duo, Shuguang
AU - Yin, Ming
AU - Shen, Huan
AU - Belmonte, Juan Carlos Izpisua
AU - Deng, Hongkui
N1 - Funding Information:
We thank L.Y. Du, X. Ch. Li, C.Y. Shan, Y. Xie, Q. Huo, Y. Tian, C. Han, S.W. Duan, L.J. Cheng, and K. Zhang for technical assistance and thank the Core Facilities at School of Life Sciences, Peking University for technical assistance, especially Lu H. X. We thank X. Zhang, Y.Q. Li, J.Y. Guan, T. Zhao, K. Liu, Y.Y. Du, and R.G. Fang for discussions in the course of the preparation of this manuscript. This work was supported by the National Key Research and Development Program of China (2016YFA0100100), the National Natural Science Foundation of China (31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Planning Project of Guangdong Province, China (2014B020226001), the Science and Technology Program of Guangzhou, China (2016B030232001), and the Ministry of Education of China (111 Project). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute and a collaborative pilot grant by the Joint Institute of Peking University Health Science Center and University of Michigan Health System. J.X. was supported in part by the Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences. C.Y.W. was supported by the National Science and Technology Support Project (2014BAI02B01). S.G.D. was supported by the CAS Key Technology Talent Program. Work in the laboratory of J.C.I.B. was supported by the G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/4/6
Y1 - 2017/4/6
N2 - Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of developmental potency and are characterized by their ability to generate all cell types of an adult organism. However, PSCs show limited contribution to the extraembryonic placental tissues in vivo. Here, we show that a chemical cocktail enables the derivation of stem cells with unique functional and molecular features from mice and humans, designated as extended pluripotent stem (EPS) cells, which are capable of chimerizing both embryonic and extraembryonic tissues. Notably, a single mouse EPS cell shows widespread chimeric contribution to both embryonic and extraembryonic lineages in vivo and permits generating single-EPS-cell-derived mice by tetraploid complementation. Furthermore, human EPS cells exhibit interspecies chimeric competency in mouse conceptuses. Our findings constitute a first step toward capturing pluripotent stem cells with extraembryonic developmental potentials in culture and open new avenues for basic and translational research.
AB - Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of developmental potency and are characterized by their ability to generate all cell types of an adult organism. However, PSCs show limited contribution to the extraembryonic placental tissues in vivo. Here, we show that a chemical cocktail enables the derivation of stem cells with unique functional and molecular features from mice and humans, designated as extended pluripotent stem (EPS) cells, which are capable of chimerizing both embryonic and extraembryonic tissues. Notably, a single mouse EPS cell shows widespread chimeric contribution to both embryonic and extraembryonic lineages in vivo and permits generating single-EPS-cell-derived mice by tetraploid complementation. Furthermore, human EPS cells exhibit interspecies chimeric competency in mouse conceptuses. Our findings constitute a first step toward capturing pluripotent stem cells with extraembryonic developmental potentials in culture and open new avenues for basic and translational research.
KW - chimeric ability
KW - embryonic and extraembryonic developmental potentials
KW - interspecies chimeric competency
KW - single-cell derived chimeras
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U2 - 10.1016/j.cell.2017.02.005
DO - 10.1016/j.cell.2017.02.005
M3 - Article
C2 - 28388409
AN - SCOPUS:85017162810
SN - 0092-8674
VL - 169
SP - 243-257.e25
JO - Cell
JF - Cell
IS - 2
ER -