Derivation of sheep embryonic stem cells under optimized conditions

Marcela Vilarino, Delia Alba Soto, Yanina Soledad Bogliotti, Leqian Yu, Yanli Zhang, Chunsheng Wang, Erika Paulson, Cuiqing Zhong, Miaohan Jin, Juan Carlos Izpisua Belmonte, Jun Wu, Pablo Juan Ross

Research output: Contribution to journalArticlepeer-review

Abstract

Until recently, it has been difficult to derive and maintain stable embryonic stem cells lines from livestock species. Sheep ESCs with characteristics similar to those described for rodents and primates have not been produced. We report the derivation of sheep ESCs under a chemically defined culture system containing fibroblast growth factor 2 (FGF2) and a tankyrase/Wnt inhibitor (IWR1). We also show that several culture conditions used for stabilizing naïve and intermediate pluripotency states in humans and mice were unsuitable to maintain ovine pluripotency in vitro. Sheep ESCs display a smooth dome-shaped colony morphology, and maintain an euploid karyotype and stable expression of pluripotency markers after more than 40 passages. We further demonstrate that IWR1 and FGF2 are essential for the maintenance of an undifferentiated state in de novo derived sheep ESCs. The derivation of stable pluripotent cell lines from sheep blastocysts represents a step forward toward understanding pluripotency regulation in livestock species and developing novel biomedical and agricultural applications.

Original languageEnglish (US)
Pages (from-to)761-772
Number of pages12
JournalReproduction
Volume160
Issue number5
DOIs
StatePublished - Nov 2020

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Fingerprint Dive into the research topics of 'Derivation of sheep embryonic stem cells under optimized conditions'. Together they form a unique fingerprint.

Cite this