Design and evaluation of inhibitors for dipeptidyl peptidase I (Cathepsin C)

Chih Min Kam, Marion G. Götz, Gretchen Koot, Michael McGuire, Dwain L Thiele, Dorothy Hudig, James C. Powers

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Dipeptidyl peptidase I (DPPI, cathepsin C) is a lysosomal cysteine protease that can activate zymogens of several different serine proteases by one step or sequential removal of dipeptides from the N-termini of the pro-protease protein substrates. To find DPPI inhibitors more suitable for cellular applications than diazomethyl ketones, we synthesized three types of inhibitors: dipeptide acyloxymethyl ketones, fluoromethyl ketones, and vinyl sulfones (VS). The acyloxymethyl ketones inhibited DPPI slowly and are moderate inhibitors of cellular DPPI. The fluoromethyl ketones were potent, but the inhibited DPPI regained activity quickly. The dipeptide vinyl sulfones were effective inhibitors for DPPI, but they also inhibited cathepsins B, H, and L weakly. The best inhibitor, Ala-Hph-VS-Ph, had a k2/KI of 2,000,000M-1s-1. The vinyl sulfones also inhibited intracellular DPPI, and for this application the more stable inhibitors exhibit better potency. We conclude that vinyl sulfones are promising inhibitors to study the intracellular functions of DPPI.

Original languageEnglish (US)
Pages (from-to)123-134
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume427
Issue number2
DOIs
StatePublished - Jul 15 2004

Keywords

  • Acyloxymethyl ketones
  • Cathepsin C
  • Cysteine protease
  • Dipeptidyl peptidase I
  • Dipeptidyl vinyl sulfones
  • Granzymes
  • Halomethyl ketones

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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