TY - JOUR
T1 - Detection and phenotypic characterization of adult neurogenesis
AU - Georg Kuhn, H. Georg
AU - Eisch, Amelia J.
AU - Spalding, Kirsty
AU - Peterson, Daniel A.
N1 - Publisher Copyright:
© 2016 Cold Spring Harbor Laboratory Press; all rights reserved.
PY - 2016/3
Y1 - 2016/3
N2 - Studies of adult neurogenesis have greatly expanded in the last decade, largely as a result of improved tools for detecting and quantifying neurogenesis. In this review,we summarize and critically evaluate detection methods for neurogenesis in mammalian and human brain tissue. Besides thymidine analog labeling, cell-cycle markers are discussed, as well as cell stage and lineage commitment markers. Use of these histological tools is critically evaluated in terms of their strengths and limitations, as well as possible artifacts. Finally, we discuss the method of radiocarbon dating for determining cell and tissue turnover in humans.
AB - Studies of adult neurogenesis have greatly expanded in the last decade, largely as a result of improved tools for detecting and quantifying neurogenesis. In this review,we summarize and critically evaluate detection methods for neurogenesis in mammalian and human brain tissue. Besides thymidine analog labeling, cell-cycle markers are discussed, as well as cell stage and lineage commitment markers. Use of these histological tools is critically evaluated in terms of their strengths and limitations, as well as possible artifacts. Finally, we discuss the method of radiocarbon dating for determining cell and tissue turnover in humans.
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U2 - 10.1101/cshperspect.a025981
DO - 10.1101/cshperspect.a025981
M3 - Article
C2 - 26931327
AN - SCOPUS:84959533742
SN - 1943-0264
VL - 8
JO - Cold Spring Harbor Perspectives in Biology
JF - Cold Spring Harbor Perspectives in Biology
IS - 3
M1 - a025981
ER -