Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR

Friedrich Hubertus Schmitz-Winnenthal, Heldgard Weckauf, Sabine Haufe, Ulf Hinz, Kaspar Z'graggen, Ernst Klar, Markus W. Büchler, Theresia Weber, Michael J. Demeure, Orlo H. Clark, Petra B. Musholt, Theresia Weber, Fiemu Nwariaku

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Abstract

Background. Metastatic disease in epithelial cancer results from tumor cell dissemination. We investigated an expression of cytokeratin 20 (CK20) by reverse transcriptase-polymerase chain reaction (RT-PCR) in differentiated (DTC) and anaplastic thyroid carcinomas (ATC) and correlated the results with TNM categories and the clinical follow-up. Methods. Tissue and blood samples of 32 patients with papillary (PTC), 17 patients with follicular (FTC), and 7 patients with ATC were obtained during operation and subjected to CK20 RT-PCR. Results. An expression of CK20 transcripts was detected in 47% of the tissue samples of PTC, 71% of the FTC, and 14% of the ATC. Patients with CK20-positive FTC had a significantly better outcome than patients with CK20-negative FTCs (P = .0016). Disseminated tumor cells were found in 9 of 22 (41%) blood samples of patients with CK20-positive carcinomas. The detection of CK20 transcripts in peripheral blood correlated with tumor categories. Four of 8 (50%) patients with DTC and circulating tumor cells developed local or distant recurrence compared with 3 of 13 (23%) patients with CK20-positive carcinomas and CK20-negative blood samples. Conclusion. Our results suggest that CK20 might be a suitable differentiation marker in thyroid carcinomas. In patients with CK20-positive tumors, those with CK20-posilive blood samples had a poorer prognosis. We suggest that patients with thyroid cancer with positive CK20 blood samples should be evaluated for further adjuvant therapies after surgery.

Original languageEnglish (US)
Pages (from-to)964-972
Number of pages9
JournalSurgery
Volume134
Issue number6
DOIs
StatePublished - Dec 2003

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Keratin-20
Reverse Transcriptase Polymerase Chain Reaction
Factor IX
Neoplasms
Thyroid Neoplasms
Anaplastic Thyroid Carcinoma
Carcinoma
Circulating Neoplastic Cells
Differentiation Antigens

ASJC Scopus subject areas

  • Surgery

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Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR. / Schmitz-Winnenthal, Friedrich Hubertus; Weckauf, Heldgard; Haufe, Sabine; Hinz, Ulf; Z'graggen, Kaspar; Klar, Ernst; Büchler, Markus W.; Weber, Theresia; Demeure, Michael J.; Clark, Orlo H.; Musholt, Petra B.; Weber, Theresia; Nwariaku, Fiemu.

In: Surgery, Vol. 134, No. 6, 12.2003, p. 964-972.

Research output: Contribution to journalArticle

Schmitz-Winnenthal, FH, Weckauf, H, Haufe, S, Hinz, U, Z'graggen, K, Klar, E, Büchler, MW, Weber, T, Demeure, MJ, Clark, OH, Musholt, PB, Weber, T & Nwariaku, F 2003, 'Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR', Surgery, vol. 134, no. 6, pp. 964-972. https://doi.org/10.1016/S0039-6060(03)00428-8
Schmitz-Winnenthal, Friedrich Hubertus ; Weckauf, Heldgard ; Haufe, Sabine ; Hinz, Ulf ; Z'graggen, Kaspar ; Klar, Ernst ; Büchler, Markus W. ; Weber, Theresia ; Demeure, Michael J. ; Clark, Orlo H. ; Musholt, Petra B. ; Weber, Theresia ; Nwariaku, Fiemu. / Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR. In: Surgery. 2003 ; Vol. 134, No. 6. pp. 964-972.
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abstract = "Background. Metastatic disease in epithelial cancer results from tumor cell dissemination. We investigated an expression of cytokeratin 20 (CK20) by reverse transcriptase-polymerase chain reaction (RT-PCR) in differentiated (DTC) and anaplastic thyroid carcinomas (ATC) and correlated the results with TNM categories and the clinical follow-up. Methods. Tissue and blood samples of 32 patients with papillary (PTC), 17 patients with follicular (FTC), and 7 patients with ATC were obtained during operation and subjected to CK20 RT-PCR. Results. An expression of CK20 transcripts was detected in 47{\%} of the tissue samples of PTC, 71{\%} of the FTC, and 14{\%} of the ATC. Patients with CK20-positive FTC had a significantly better outcome than patients with CK20-negative FTCs (P = .0016). Disseminated tumor cells were found in 9 of 22 (41{\%}) blood samples of patients with CK20-positive carcinomas. The detection of CK20 transcripts in peripheral blood correlated with tumor categories. Four of 8 (50{\%}) patients with DTC and circulating tumor cells developed local or distant recurrence compared with 3 of 13 (23{\%}) patients with CK20-positive carcinomas and CK20-negative blood samples. Conclusion. Our results suggest that CK20 might be a suitable differentiation marker in thyroid carcinomas. In patients with CK20-positive tumors, those with CK20-posilive blood samples had a poorer prognosis. We suggest that patients with thyroid cancer with positive CK20 blood samples should be evaluated for further adjuvant therapies after surgery.",
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T1 - Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR

AU - Schmitz-Winnenthal, Friedrich Hubertus

AU - Weckauf, Heldgard

AU - Haufe, Sabine

AU - Hinz, Ulf

AU - Z'graggen, Kaspar

AU - Klar, Ernst

AU - Büchler, Markus W.

AU - Weber, Theresia

AU - Demeure, Michael J.

AU - Clark, Orlo H.

AU - Musholt, Petra B.

AU - Weber, Theresia

AU - Nwariaku, Fiemu

PY - 2003/12

Y1 - 2003/12

N2 - Background. Metastatic disease in epithelial cancer results from tumor cell dissemination. We investigated an expression of cytokeratin 20 (CK20) by reverse transcriptase-polymerase chain reaction (RT-PCR) in differentiated (DTC) and anaplastic thyroid carcinomas (ATC) and correlated the results with TNM categories and the clinical follow-up. Methods. Tissue and blood samples of 32 patients with papillary (PTC), 17 patients with follicular (FTC), and 7 patients with ATC were obtained during operation and subjected to CK20 RT-PCR. Results. An expression of CK20 transcripts was detected in 47% of the tissue samples of PTC, 71% of the FTC, and 14% of the ATC. Patients with CK20-positive FTC had a significantly better outcome than patients with CK20-negative FTCs (P = .0016). Disseminated tumor cells were found in 9 of 22 (41%) blood samples of patients with CK20-positive carcinomas. The detection of CK20 transcripts in peripheral blood correlated with tumor categories. Four of 8 (50%) patients with DTC and circulating tumor cells developed local or distant recurrence compared with 3 of 13 (23%) patients with CK20-positive carcinomas and CK20-negative blood samples. Conclusion. Our results suggest that CK20 might be a suitable differentiation marker in thyroid carcinomas. In patients with CK20-positive tumors, those with CK20-posilive blood samples had a poorer prognosis. We suggest that patients with thyroid cancer with positive CK20 blood samples should be evaluated for further adjuvant therapies after surgery.

AB - Background. Metastatic disease in epithelial cancer results from tumor cell dissemination. We investigated an expression of cytokeratin 20 (CK20) by reverse transcriptase-polymerase chain reaction (RT-PCR) in differentiated (DTC) and anaplastic thyroid carcinomas (ATC) and correlated the results with TNM categories and the clinical follow-up. Methods. Tissue and blood samples of 32 patients with papillary (PTC), 17 patients with follicular (FTC), and 7 patients with ATC were obtained during operation and subjected to CK20 RT-PCR. Results. An expression of CK20 transcripts was detected in 47% of the tissue samples of PTC, 71% of the FTC, and 14% of the ATC. Patients with CK20-positive FTC had a significantly better outcome than patients with CK20-negative FTCs (P = .0016). Disseminated tumor cells were found in 9 of 22 (41%) blood samples of patients with CK20-positive carcinomas. The detection of CK20 transcripts in peripheral blood correlated with tumor categories. Four of 8 (50%) patients with DTC and circulating tumor cells developed local or distant recurrence compared with 3 of 13 (23%) patients with CK20-positive carcinomas and CK20-negative blood samples. Conclusion. Our results suggest that CK20 might be a suitable differentiation marker in thyroid carcinomas. In patients with CK20-positive tumors, those with CK20-posilive blood samples had a poorer prognosis. We suggest that patients with thyroid cancer with positive CK20 blood samples should be evaluated for further adjuvant therapies after surgery.

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