Detection of different grade of cancerous cell regarding their impedance

S. Abbasi, M. Janmaleki, M. Kazemi Moghadam, M. Abdolahad, S. Mohajerzadeh, H. Peirovi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

There is an increasing demand for more practical while being easy to perform methods to study cell behavior. Here a developed Electric Cell-substrate Impedance Sensor (ECIS) biosensor has been utilized to study different grade of cancerous cells. ECIS biosensor consists of a number pair of gold electrodes in micrometer scale on glass substrate. The impedance is measured by NI DAQ. An equivalent electrical circuit has been developed to model the ECIS behavior in the cell culture. The size of the circuit elements has been calculated by curve fitting method in MATLAB. The impedance of two grades of human colon cancerous cell, HT29 and SW48 has been studied. Previous studies have shown that the expression of adhering molecule has been affected in the cancerous cell. Regarding this studies, cancer can change the cell properties. This changes can occurred in the membrane properties as well as expression of a series of molecule. Impedance spectrometry can detect the changes in cell size as well as adhering properties of cell caused by cancer. Developing this method, it can be used to detect the grade of cancer.

Original languageEnglish (US)
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Pages1416-1419
Number of pages4
DOIs
StatePublished - 2013
Externally publishedYes
EventWorld Congress on Medical Physics and Biomedical Engineering - Beijing, China
Duration: May 26 2012May 31 2012

Publication series

NameIFMBE Proceedings
Volume39 IFMBE
ISSN (Print)1680-0737

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering
Country/TerritoryChina
CityBeijing
Period5/26/125/31/12

Keywords

  • adhering molecule
  • ECIS biosensor
  • grade of cancerous cell
  • Impedance

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Detection of different grade of cancerous cell regarding their impedance'. Together they form a unique fingerprint.

Cite this