Detection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies

Harri Saxen, Jaana Vuopio-Varkila, John Luk, Alf Lindberg, Alois Lang, Franco Di Padova, Stanley J. Cryz, Jussi Mertsola, George H. McCracken, Eric J. Hansen

Research output: Contribution to journalArticle

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Abstract

The immunolimulus (IML) assay system uses solid-phase endotoxin antibodies to capture lipopolysaccharide (LPS), which is then quantified by a modification of the chromogenic limulus amebocyte lysate (CLAL) method. Monoclonal antibodies (MAbs) reactive with selected O antigen serotypes of Escherichia coli (O18) and Salmonella typhimurium (O-9,12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals. A murine MAb that was broadly cross-reactive with E. coli, Salmonella, and Shigella endotoxins also proved to be highly effective in the IML assay for capturing LPS molecules from both E. coli and S. typhimurium strains. These results indicate that IML assays can detect smooth-type enterobacterial endotoxins in plasma and suggest that such assays have potential for use in the rapid diagnosis of sepsis and endotoxemia caused by different enterobacterial species.

Original languageEnglish (US)
Pages (from-to)393-399
Number of pages7
JournalJournal of Infectious Diseases
Volume168
Issue number2
StatePublished - Aug 1993

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Endotoxemia
Endotoxins
Lipopolysaccharides
Salmonella typhimurium
Escherichia coli
Antibodies
Horseshoe Crabs
O Antigens
Shigella
Sepsis
Monoclonal Antibodies
Serogroup

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Detection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies. / Saxen, Harri; Vuopio-Varkila, Jaana; Luk, John; Lindberg, Alf; Lang, Alois; Di Padova, Franco; Cryz, Stanley J.; Mertsola, Jussi; McCracken, George H.; Hansen, Eric J.

In: Journal of Infectious Diseases, Vol. 168, No. 2, 08.1993, p. 393-399.

Research output: Contribution to journalArticle

Saxen, H, Vuopio-Varkila, J, Luk, J, Lindberg, A, Lang, A, Di Padova, F, Cryz, SJ, Mertsola, J, McCracken, GH & Hansen, EJ 1993, 'Detection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies', Journal of Infectious Diseases, vol. 168, no. 2, pp. 393-399.
Saxen, Harri ; Vuopio-Varkila, Jaana ; Luk, John ; Lindberg, Alf ; Lang, Alois ; Di Padova, Franco ; Cryz, Stanley J. ; Mertsola, Jussi ; McCracken, George H. ; Hansen, Eric J. / Detection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies. In: Journal of Infectious Diseases. 1993 ; Vol. 168, No. 2. pp. 393-399.
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