TY - JOUR
T1 - Detection of experimental Haemophilus influenzae type b bacteremia and endotoxemia by means of an immunolimulus assay
AU - Mertsola, Jussi
AU - Cope, Leslie D.
AU - Munford, Robert S.
AU - McCracken, George H.
AU - Hansen, Eric J.
N1 - Funding Information:
Received 8 November 1990; revised I April 1991. Financial support: Public Health Service (HD-27766 to E.J.H.); J.M. received partial support from the Academy of Finland and the Paulo Foundation. Reprints or correspondence: Dr. Eric J. Hansen, Department ofMicrobiology. University ofTexas Southwestern Medical Center. 5323 Harry Hines Blvd., Dallas, TX 75235-9048. * Present address: National Public Health Institute, Turku, Finland.
PY - 1991/8
Y1 - 1991/8
N2 - The rapid quantitation of bacteria in blood was achieved by using a novel assay method for gram-negative bacterial lipopolysaccharide (endotoxin, LPS). The assay involves the capture of specific LPS onto microtiter plates by means of monoclonal antibodies directed against the oligosaccharide region of the LPS, followed by detection of the bound LPS by a chromogenic Limulus amebocyte lysate (LAL) system. This immunolimulus (IML) assay combines the specificity of monoclonal antibodies with the sensitivity of the LAL system to achieve the first specific, sensitive quantitation of bioactive endotoxin in plasma. In the animal model tested, Haemophilus influenzae type b (Hib) bacteremia in infant rats, there was a strong correlation between IML results and the concentration of Hib colony-forming units in blood samples (r = .845, P < .001). Using antibodies with appropriate specificities, this approach should be useful for rapid detection of a wide range of gram-negative bacteria and endotoxins in blood.
AB - The rapid quantitation of bacteria in blood was achieved by using a novel assay method for gram-negative bacterial lipopolysaccharide (endotoxin, LPS). The assay involves the capture of specific LPS onto microtiter plates by means of monoclonal antibodies directed against the oligosaccharide region of the LPS, followed by detection of the bound LPS by a chromogenic Limulus amebocyte lysate (LAL) system. This immunolimulus (IML) assay combines the specificity of monoclonal antibodies with the sensitivity of the LAL system to achieve the first specific, sensitive quantitation of bioactive endotoxin in plasma. In the animal model tested, Haemophilus influenzae type b (Hib) bacteremia in infant rats, there was a strong correlation between IML results and the concentration of Hib colony-forming units in blood samples (r = .845, P < .001). Using antibodies with appropriate specificities, this approach should be useful for rapid detection of a wide range of gram-negative bacteria and endotoxins in blood.
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M3 - Article
C2 - 1856484
AN - SCOPUS:0025879325
SN - 0022-1899
VL - 164
SP - 353
EP - 358
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -