Detection of Haemophilus ducreyi lipooligosaccharide by means of an immunolimulus assay

Eric J. Hansen, Sheryl R. Lumbley, Harri Saxen, Kevin Kern, Leslie D. Cope, Justin D. Radolf

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A murine monoclonal antibody (MAb) directed against a surface-exposed epitope of the lipooligosaccharide (LOS) of Haemophilus ducreyi strain 35000 was shown to be reactive with all 37 strains of this pathogen tested in a colony blot-radioimmunoassay. The LOS epitope bound by this MAb appeared to be stably expressed by H. ducreyi growing in vitro. The use of this MAb in the immunolimulus system revealed that it could detect purified H. ducreyi LOS at a level of 25 pg/ml. Similarly, this immunolimulus system could detect as few as 1000 colony forming units of in vitro-grown H. ducreyi cells per ml of buffer. When this MAb was utilized in the immunolimulus system together with lesion material from rabbits infected with two different H. ducreyi strains, a positive reaction was obtained with every sample tested, even when no viable organisms were present in the lesion material. In contrast, this MAb yielded consistently negative results when used in the immunolimulus system with lesion material from animals infected with Staphylococcus aureus.

Original languageEnglish (US)
Pages (from-to)225-235
Number of pages11
JournalJournal of Immunological Methods
Volume185
Issue number2
DOIs
StatePublished - 1995

Keywords

  • Chancroid
  • Endotoxin
  • Haemophilus ducreyi
  • Lipooligosaccharide
  • detection of

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Hansen, E. J., Lumbley, S. R., Saxen, H., Kern, K., Cope, L. D., & Radolf, J. D. (1995). Detection of Haemophilus ducreyi lipooligosaccharide by means of an immunolimulus assay. Journal of Immunological Methods, 185(2), 225-235. https://doi.org/10.1016/0022-1759(95)00118-T