Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction

A. M H Nguyen, L. Engstrand, R. M. Genta, D. Y. Graham, F. A K El-Zaatari

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

To investigate whether the oral cavity is a potential reservoir and possible sanctuary for Helicobacter pylori, supragingival and subgingival plaques were analyzed by a Helicobacter genus-specific reverse transcriptase- polymerase chain reaction based on the sequence data of H. pylori 16S rRNA. The amplified 500-bp DNA fragment was identified by ethidium bromide staining after agarose gel electrophoresis and by Southern hybridization. Twenty-five dyspeptic patients were studied. Histologic examination of gastric biopsy specimens revealed that 18 had H. pylori gastritis and 7 did not. For seven of the 18 (38.8%) patients with proven H. pylori gastritis, H. pylori was also identified in their dental plaque. None of the patients without H. pylori gastritis had H. pylori in their dental plaque. The detection of H. pylori in dental plaque suggests that this H. pylori colonization is not restricted to the gastric mucosa and that this ecological niche may serve as a possible sanctuary which may be responsible for reinoculation of the stomach after topical anti-H. pylori therapies such as bismuth.

Original languageEnglish (US)
Pages (from-to)783-787
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number4
StatePublished - 1993

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Dental Plaque
Helicobacter pylori
Reverse Transcription
Polymerase Chain Reaction
Gastritis
Stomach
Helicobacter
Bismuth
Ethidium
Agar Gel Electrophoresis
Gastric Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Mouth
Staining and Labeling
Biopsy

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Nguyen, A. M. H., Engstrand, L., Genta, R. M., Graham, D. Y., & El-Zaatari, F. A. K. (1993). Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. Journal of Clinical Microbiology, 31(4), 783-787.

Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. / Nguyen, A. M H; Engstrand, L.; Genta, R. M.; Graham, D. Y.; El-Zaatari, F. A K.

In: Journal of Clinical Microbiology, Vol. 31, No. 4, 1993, p. 783-787.

Research output: Contribution to journalArticle

Nguyen, AMH, Engstrand, L, Genta, RM, Graham, DY & El-Zaatari, FAK 1993, 'Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction', Journal of Clinical Microbiology, vol. 31, no. 4, pp. 783-787.
Nguyen, A. M H ; Engstrand, L. ; Genta, R. M. ; Graham, D. Y. ; El-Zaatari, F. A K. / Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 4. pp. 783-787.
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