TY - JOUR
T1 - Detection of hepatitis B virus transcripts in patients with chronic liver disease
AU - Pasquinelli, Claudio
AU - Melegari, Margherita
AU - Villa, Erica
AU - Seidenari, Michela
AU - Scaglioni, Pier Paolo
AU - Tiribelli, Claudio
AU - Crocé, Lory S.
AU - Manenti, Federico
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990/3
Y1 - 1990/3
N2 - Hepatitis B virus (HBV) transcription was studied by Northern blot analysis on total cellular RNA purified from liver biopsies in 70 patients with chronic liver disease (24 HBsAg positive, 15 antiHBs and/or antiHBc positive, 31 HBV negative). No transcripts were found in the HBV negative and in the antiHBs and/or antiHBc positive patients. In the others, three major RNA species were identified: i. a 3.5 kb transcript corresponding to the RNA pregenome; ii. 2.4-2.1 kb transcript corresponding to the s and preS1 gene RNA; iii. lower molecular weight species. All three forms were present simultaneously only in patients with active viral replication, with a strict relation between the presence of the 3.5 kb RNA in the liver and serum HBV-DNA. In conclusion, Northern blot analysis can easily be performed to study viral replication and it can contribute to a better understanding of the molecular processes underlying HBV infection and leading to liver disease in man.
AB - Hepatitis B virus (HBV) transcription was studied by Northern blot analysis on total cellular RNA purified from liver biopsies in 70 patients with chronic liver disease (24 HBsAg positive, 15 antiHBs and/or antiHBc positive, 31 HBV negative). No transcripts were found in the HBV negative and in the antiHBs and/or antiHBc positive patients. In the others, three major RNA species were identified: i. a 3.5 kb transcript corresponding to the RNA pregenome; ii. 2.4-2.1 kb transcript corresponding to the s and preS1 gene RNA; iii. lower molecular weight species. All three forms were present simultaneously only in patients with active viral replication, with a strict relation between the presence of the 3.5 kb RNA in the liver and serum HBV-DNA. In conclusion, Northern blot analysis can easily be performed to study viral replication and it can contribute to a better understanding of the molecular processes underlying HBV infection and leading to liver disease in man.
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U2 - 10.1016/0168-8278(90)90049-W
DO - 10.1016/0168-8278(90)90049-W
M3 - Article
C2 - 2332589
AN - SCOPUS:0025319439
SN - 0168-8278
VL - 10
SP - 180
EP - 185
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 2
ER -