Detection of hepatitis B virus transcripts in patients with chronic liver disease

Claudio Pasquinelli, Margherita Melegari, Erica Villa, Michela Seidenari, Pier Paolo Scaglioni, Claudio Tiribelli, Lory S. Crocé, Federico Manenti

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Abstract

Hepatitis B virus (HBV) transcription was studied by Northern blot analysis on total cellular RNA purified from liver biopsies in 70 patients with chronic liver disease (24 HBsAg positive, 15 antiHBs and/or antiHBc positive, 31 HBV negative). No transcripts were found in the HBV negative and in the antiHBs and/or antiHBc positive patients. In the others, three major RNA species were identified: i. a 3.5 kb transcript corresponding to the RNA pregenome; ii. 2.4-2.1 kb transcript corresponding to the s and preS1 gene RNA; iii. lower molecular weight species. All three forms were present simultaneously only in patients with active viral replication, with a strict relation between the presence of the 3.5 kb RNA in the liver and serum HBV-DNA. In conclusion, Northern blot analysis can easily be performed to study viral replication and it can contribute to a better understanding of the molecular processes underlying HBV infection and leading to liver disease in man.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalJournal of Hepatology
Volume10
Issue number2
DOIs
StatePublished - Mar 1990

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ASJC Scopus subject areas

  • Hepatology

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Pasquinelli, C., Melegari, M., Villa, E., Seidenari, M., Scaglioni, P. P., Tiribelli, C., Crocé, L. S., & Manenti, F. (1990). Detection of hepatitis B virus transcripts in patients with chronic liver disease. Journal of Hepatology, 10(2), 180-185. https://doi.org/10.1016/0168-8278(90)90049-W