Detection of phase variation in expression of proteins involved in hemoglobin and hemoglobin-haptoglobin binding by nontypeable Haemophilus influenzae

Leslie D. Cope, Zbynek Hrkal, Eric J. Hansen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Haemophilus influenzae can utilize different protein-bound forms of heme for growth in vitro. A previous study (I. Maciver, J. L. Latimer, H. H. Liem, U. Muller-Eberhard, Z. Hrkal, and E. J. Hansen. Infect. Immun. 64:3703-3712, 1996) indicated that nontypeable H. influenzae (NTHI) strain TN106 expressed a protein that bound hemoglobin-haptoglobin and was encoded by an open reading frame (ORF) that contained a CCAA nucleotide repeat. Southern blot analysis revealed that several NTHI strains contained between three and five chromosomal DNA fragments that bound an oligonucleotide probe for CCAA repeats. Three ORFs containing CCAA repeats were identified in NTHI strain N182; two of these ORFs were arranged in tandem. The use of translational fusions involving these three ORFs and the β-lactamase gene from pBR322 revealed that these three ORFs, designated hgbA, hgbB, and hgbC, encoded proteins that could bind hemoglobin, hemoglobin-haptoglobin, or both compounds. Monoclonal antibodies (MAbs) specific for the HgbA, HgbB, and HgbC proteins were produced by immunizing mice with synthetic peptides unique to each protein. Both HgbA and HgbB were readily detected by Western blot analysis in N182 cells grown in the presence of hemoglobin as the sole source of heme, whereas expression of HgbC was found to be much less abundant than that of HgbA and HgbB. The use of these MAbs in a colony blot radioimmunoassay analysis revealed that expression of both HgbA and HgbB was subject to phase variation. PCR and nucleotide sequence analysis were used in conjunction with Western blot analyses to demonstrate that this phase variation involved the CCAA repeats in the hgbA and hgbB ORFs.

Original languageEnglish (US)
Pages (from-to)4092-4101
Number of pages10
JournalInfection and Immunity
Volume68
Issue number7
DOIs
StatePublished - Jul 2000

Fingerprint

Haptoglobins
Haemophilus influenzae
Open Reading Frames
Hemoglobins
Proteins
Heme
Western Blotting
Monoclonal Antibodies
Oligonucleotide Probes
Southern Blotting
Radioimmunoassay
Sequence Analysis
Nucleotides
Polymerase Chain Reaction
Peptides
DNA
Growth
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Detection of phase variation in expression of proteins involved in hemoglobin and hemoglobin-haptoglobin binding by nontypeable Haemophilus influenzae. / Cope, Leslie D.; Hrkal, Zbynek; Hansen, Eric J.

In: Infection and Immunity, Vol. 68, No. 7, 07.2000, p. 4092-4101.

Research output: Contribution to journalArticle

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