Detection of teichoic acid antibodies in children with staphylococcal infections.

U. Thisyakorn, S. Shelton, T. Y. Lin, G. H. McCracken, J. D. Nelson

Research output: Contribution to journalArticle

Abstract

The presence of serum antibodies to teichoic acid was evaluated by gel diffusion and enzyme-linked immunosorbent assay in 14 patients with deep-seated staphylococcal infection, in 5 patients with superficial staphylococcal infections, in 10 patients with Gram-positive infections other than staphylococcal and in 12 age-matched, uninfected patients. Serum samples were obtained on admission and serially each week during hospitalization. Teichoic acid antibodies were detected by gel diffusion in only 5 of 14 patients with deep-seated staphylococcal infections, in 1 of 10 patients with other Gram-positive infections and in none of the other patients. With the enzyme-linked immunosorbent assay method all patients with deep-seated staphylococcal infections had concentrations of teichoic acid antibodies of 1:1600 or greater, and these titers were significantly larger than those in the other groups of patients. Using a titer of 1:3200 or greater as a diagnostic level in children with deep-seated Staphylococcus aureus infections, the sensitivity was 93% and the specificity was 89%. For all staphylococcal infections the sensitivity was 79% and the specificity was 96%.

Original languageEnglish (US)
Pages (from-to)222-225
Number of pages4
JournalPediatric Infectious Disease
Volume3
Issue number3
StatePublished - May 1984

Fingerprint

Teichoic Acids
Staphylococcal Infections
Antibodies
Gels
Enzyme-Linked Immunosorbent Assay
Infection
Serum
Staphylococcus aureus
Hospitalization

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of teichoic acid antibodies in children with staphylococcal infections. / Thisyakorn, U.; Shelton, S.; Lin, T. Y.; McCracken, G. H.; Nelson, J. D.

In: Pediatric Infectious Disease, Vol. 3, No. 3, 05.1984, p. 222-225.

Research output: Contribution to journalArticle

@article{93a775f13a2a43edb4d3ec907e9c03b8,
title = "Detection of teichoic acid antibodies in children with staphylococcal infections.",
abstract = "The presence of serum antibodies to teichoic acid was evaluated by gel diffusion and enzyme-linked immunosorbent assay in 14 patients with deep-seated staphylococcal infection, in 5 patients with superficial staphylococcal infections, in 10 patients with Gram-positive infections other than staphylococcal and in 12 age-matched, uninfected patients. Serum samples were obtained on admission and serially each week during hospitalization. Teichoic acid antibodies were detected by gel diffusion in only 5 of 14 patients with deep-seated staphylococcal infections, in 1 of 10 patients with other Gram-positive infections and in none of the other patients. With the enzyme-linked immunosorbent assay method all patients with deep-seated staphylococcal infections had concentrations of teichoic acid antibodies of 1:1600 or greater, and these titers were significantly larger than those in the other groups of patients. Using a titer of 1:3200 or greater as a diagnostic level in children with deep-seated Staphylococcus aureus infections, the sensitivity was 93{\%} and the specificity was 89{\%}. For all staphylococcal infections the sensitivity was 79{\%} and the specificity was 96{\%}.",
author = "U. Thisyakorn and S. Shelton and Lin, {T. Y.} and McCracken, {G. H.} and Nelson, {J. D.}",
year = "1984",
month = "5",
language = "English (US)",
volume = "3",
pages = "222--225",
journal = "Pediatric Infectious Disease Journal",
issn = "0891-3668",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Detection of teichoic acid antibodies in children with staphylococcal infections.

AU - Thisyakorn, U.

AU - Shelton, S.

AU - Lin, T. Y.

AU - McCracken, G. H.

AU - Nelson, J. D.

PY - 1984/5

Y1 - 1984/5

N2 - The presence of serum antibodies to teichoic acid was evaluated by gel diffusion and enzyme-linked immunosorbent assay in 14 patients with deep-seated staphylococcal infection, in 5 patients with superficial staphylococcal infections, in 10 patients with Gram-positive infections other than staphylococcal and in 12 age-matched, uninfected patients. Serum samples were obtained on admission and serially each week during hospitalization. Teichoic acid antibodies were detected by gel diffusion in only 5 of 14 patients with deep-seated staphylococcal infections, in 1 of 10 patients with other Gram-positive infections and in none of the other patients. With the enzyme-linked immunosorbent assay method all patients with deep-seated staphylococcal infections had concentrations of teichoic acid antibodies of 1:1600 or greater, and these titers were significantly larger than those in the other groups of patients. Using a titer of 1:3200 or greater as a diagnostic level in children with deep-seated Staphylococcus aureus infections, the sensitivity was 93% and the specificity was 89%. For all staphylococcal infections the sensitivity was 79% and the specificity was 96%.

AB - The presence of serum antibodies to teichoic acid was evaluated by gel diffusion and enzyme-linked immunosorbent assay in 14 patients with deep-seated staphylococcal infection, in 5 patients with superficial staphylococcal infections, in 10 patients with Gram-positive infections other than staphylococcal and in 12 age-matched, uninfected patients. Serum samples were obtained on admission and serially each week during hospitalization. Teichoic acid antibodies were detected by gel diffusion in only 5 of 14 patients with deep-seated staphylococcal infections, in 1 of 10 patients with other Gram-positive infections and in none of the other patients. With the enzyme-linked immunosorbent assay method all patients with deep-seated staphylococcal infections had concentrations of teichoic acid antibodies of 1:1600 or greater, and these titers were significantly larger than those in the other groups of patients. Using a titer of 1:3200 or greater as a diagnostic level in children with deep-seated Staphylococcus aureus infections, the sensitivity was 93% and the specificity was 89%. For all staphylococcal infections the sensitivity was 79% and the specificity was 96%.

UR - http://www.scopus.com/inward/record.url?scp=0021436670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021436670&partnerID=8YFLogxK

M3 - Article

C2 - 6429647

AN - SCOPUS:0021436670

VL - 3

SP - 222

EP - 225

JO - Pediatric Infectious Disease Journal

JF - Pediatric Infectious Disease Journal

SN - 0891-3668

IS - 3

ER -